Shuttle vectors for genetic manipulations in Ustilago maydis.

TitleShuttle vectors for genetic manipulations in Ustilago maydis.
Publication TypeJournal Article
Year of Publication2000
AuthorsKojic M, Holloman WK
JournalCan J Microbiol
Date Published2000 Apr
KeywordsAnti-Bacterial Agents, Carboxin, Cinnamates, Drug Resistance, Microbial, Fungicides, Industrial, Genetic Vectors, Gentamicins, Hygromycin B, Microbial Sensitivity Tests, Plasmids, Streptothricins, Ustilago

Shuttle vectors with new or improved features were constructed to enable facile genetic manipulations in the plant pathogen Ustilago maydis. Sets of plasmids selectable in media containing geneticin, carboxin, nourseothricin, or hygromycin, able to replicate autonomously, to transform U. maydis by integration, and to express foreign genes under control of the homologous glyceraldehyde-3-phosphate dehydrogenase promoter, were built upon a common pUC19 vector backbone. This permits a large number of choices for a cloning site, blue/white screening for recombinant plasmids, rapid transfer of a cloned DNA fragment between plasmids, and choice of several dominant drug-resistance markers for selection in U. maydis.

Alternate JournalCan. J. Microbiol.
PubMed ID10779869
Grant ListGM42482 / GM / NIGMS NIH HHS / United States

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