For COVID-19 vaccine updates, please review our information guide. For patient eligibility and scheduling availability, please visit VaccineTogetherNY.org.

Shuttle vectors for genetic manipulations in Ustilago maydis.

TitleShuttle vectors for genetic manipulations in Ustilago maydis.
Publication TypeJournal Article
Year of Publication2000
AuthorsKojic M, Holloman WK
JournalCan J Microbiol
Volume46
Issue4
Pagination333-8
Date Published2000 Apr
ISSN0008-4166
KeywordsAnti-Bacterial Agents, Carboxin, Cinnamates, Drug Resistance, Microbial, Fungicides, Industrial, Genetic Vectors, Gentamicins, Hygromycin B, Microbial Sensitivity Tests, Plasmids, Streptothricins, Ustilago
Abstract

Shuttle vectors with new or improved features were constructed to enable facile genetic manipulations in the plant pathogen Ustilago maydis. Sets of plasmids selectable in media containing geneticin, carboxin, nourseothricin, or hygromycin, able to replicate autonomously, to transform U. maydis by integration, and to express foreign genes under control of the homologous glyceraldehyde-3-phosphate dehydrogenase promoter, were built upon a common pUC19 vector backbone. This permits a large number of choices for a cloning site, blue/white screening for recombinant plasmids, rapid transfer of a cloned DNA fragment between plasmids, and choice of several dominant drug-resistance markers for selection in U. maydis.

Alternate JournalCan. J. Microbiol.
PubMed ID10779869
Grant ListGM42482 / GM / NIGMS NIH HHS / United States

Weill Cornell Medicine Microbiology and Immunology 1300 York Avenue, Box 62 New York, NY 10065 Phone: (212) 746-6505 Fax: (212) 746-8587