| Title | A glutamate-alanine-leucine (EAL) domain protein of Salmonella controls bacterial survival in mice, antioxidant defence and killing of macrophages: role of cyclic diGMP. |
| Publication Type | Journal Article |
| Year of Publication | 2005 |
| Authors | Hisert KB, MacCoss M, Shiloh MU, K Darwin H, Singh S, Jones RA, Ehrt S, Zhang Z, Gaffney BL, Gandotra S, Holden DW, Murray D, Nathan C |
| Journal | Mol Microbiol |
| Volume | 56 |
| Issue | 5 |
| Pagination | 1234-45 |
| Date Published | 2005 Jun |
| ISSN | 0950-382X |
| Keywords | Amino Acid Sequence, Animals, Anti-Bacterial Agents, Bacterial Proteins, Cyclic GMP, DNA Transposable Elements, Hydrogen Peroxide, Macrophages, Mice, Molecular Sequence Data, Mutagenesis, Insertional, Salmonella typhimurium, Sequence Alignment |
| Abstract | Signature-tagged transposon mutagenesis of Salmonella with differential recovery from wild-type and immunodeficient mice revealed that the gene here named cdgR[for c-diguanylate (c-diGMP) regulator] is required for the bacterium to resist host phagocyte oxidase in vivo. CdgR consists solely of a glutamate-alanine-leucine (EAL) domain, a predicted cyclic diGMP (c-diGMP) phosphodiesterase. Disruption of cdgR decreased bacterial resistance to hydrogen peroxide and accelerated bacterial killing of macrophages. An ultrasensitive assay revealed c-diGMP in wild-type Salmonella with increased levels in the CdgR-deficient mutant. Thus, besides its known role in regulating cellulose synthesis and biofilm formation, bacterial c-diGMP also regulates host-pathogen interactions involving antioxidant defence and cytotoxicity. |
| DOI | 10.1111/j.1365-2958.2005.04632.x |
| Alternate Journal | Mol Microbiol |
| PubMed ID | 15882417 |
| Grant List | EB002809 / EB / NIBIB NIH HHS / United States GM07739 / GM / NIGMS NIH HHS / United States HL61241 / HL / NHLBI NIH HHS / United States |
Submitted by jom4013 on December 3, 2020 - 3:26pm