For COVID-19 vaccine updates, please review our information guide. For patient eligibility and scheduling availability, please visit VaccineTogetherNY.org.

A yeast protein that influences the chromatin structure of UASG and functions as a powerful auxiliary gene activator.

TitleA yeast protein that influences the chromatin structure of UASG and functions as a powerful auxiliary gene activator.
Publication TypeJournal Article
Year of Publication1990
AuthorsChasman DI, Lue NF, Buchman AR, LaPointe JW, Lorch Y, Kornberg RD
JournalGenes Dev
Volume4
Issue4
Pagination503-14
Date Published1990 Apr
ISSN0890-9369
KeywordsBase Sequence, Chromatin, Chromatography, Affinity, DNA-Binding Proteins, Fungal Proteins, Gene Expression Regulation, Fungal, Genes, Fungal, Molecular Sequence Data, Regulatory Sequences, Nucleic Acid, Transcription Factors, Transcription, Genetic, Yeasts
Abstract

GRF2, an abundant yeast protein of Mr approximately 127,000, binds to the GAL upstream activating sequence (UASG) and creates a nucleosome-free region of approximately 230 bp. Purified GRF2 binds to sequences found in many other UASs, in the 35S rRNA enhancer, at centromeres, and at telomeres. Although GRF2 stimulates transcription only slightly on its own, it combines with a neighboring weak activator to give as much as a 170-fold enhancement. This effect of GRF2 is strongly distance-dependent, declining by 85% when 22 bp is interposed between the GRF2 and neighboring activator sites.

DOI10.1101/gad.4.4.503
Alternate JournalGenes Dev
PubMed ID2361590
Grant ListGM36659 / GM / NIGMS NIH HHS / United States

Weill Cornell Medicine Microbiology and Immunology 1300 York Avenue, Box 62 New York, NY 10065 Phone: (212) 746-6505 Fax: (212) 746-8587