Submitted by jom4013 on December 3, 2020 - 4:18pm
| Title | A yeast protein that influences the chromatin structure of UASG and functions as a powerful auxiliary gene activator. |
| Publication Type | Journal Article |
| Year of Publication | 1990 |
| Authors | Chasman DI, Lue NF, Buchman AR, LaPointe JW, Lorch Y, Kornberg RD |
| Journal | Genes Dev |
| Volume | 4 |
| Issue | 4 |
| Pagination | 503-14 |
| Date Published | 1990 Apr |
| ISSN | 0890-9369 |
| Keywords | Base Sequence, Chromatin, Chromatography, Affinity, DNA-Binding Proteins, Fungal Proteins, Gene Expression Regulation, Fungal, Genes, Fungal, Molecular Sequence Data, Regulatory Sequences, Nucleic Acid, Transcription Factors, Transcription, Genetic, Yeasts |
| Abstract | GRF2, an abundant yeast protein of Mr approximately 127,000, binds to the GAL upstream activating sequence (UASG) and creates a nucleosome-free region of approximately 230 bp. Purified GRF2 binds to sequences found in many other UASs, in the 35S rRNA enhancer, at centromeres, and at telomeres. Although GRF2 stimulates transcription only slightly on its own, it combines with a neighboring weak activator to give as much as a 170-fold enhancement. This effect of GRF2 is strongly distance-dependent, declining by 85% when 22 bp is interposed between the GRF2 and neighboring activator sites. |
| DOI | 10.1101/gad.4.4.503 |
| Alternate Journal | Genes Dev |
| PubMed ID | 2361590 |
| Grant List | GM36659 / GM / NIGMS NIH HHS / United States |