UDP-N-acetylglucosamine 1-carboxyvinyl-transferase from Acinetobacter calcoaceticus.

TitleUDP-N-acetylglucosamine 1-carboxyvinyl-transferase from Acinetobacter calcoaceticus.
Publication TypeJournal Article
Year of Publication1994
AuthorsEhrt S, Hillen W
JournalFEMS Microbiol Lett
Date Published1994 Apr 01
KeywordsAcinetobacter calcoaceticus, Alkyl and Aryl Transferases, Amino Acid Sequence, Base Sequence, DNA-Binding Proteins, DNA-Directed RNA Polymerases, Drug Resistance, Microbial, Fosfomycin, Gene Expression, Genetic Complementation Test, Molecular Sequence Data, Open Reading Frames, RNA Polymerase Sigma 54, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Sigma Factor, Transferases

We have analyzed the sequence downstream of rpoN from Acinetobacter calcoaceticus and identified an open reading frame encoding a protein with high similarity to UDP-N-acetylglucosamine 1-carboxyvinyl-transferase (MurZ). Multicopy plasmids encoding this enzyme conferred phosphomycin resistance to A. calcoaceticus. The polar effect of a rpoN mutation on the phosphomycin resistance level suggests that murZ is, in part, cotranscribed with rpoN. These observations confirm that A. calcoaceticus represents the first exception from a conserved genetic context of rpoN observed in several other Gram-negative bacteria.

Alternate JournalFEMS Microbiol Lett
PubMed ID8181716

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