Submitted by jom4013 on December 3, 2020 - 3:25pm
| Title | UDP-N-acetylglucosamine 1-carboxyvinyl-transferase from Acinetobacter calcoaceticus. |
| Publication Type | Journal Article |
| Year of Publication | 1994 |
| Authors | Ehrt S, Hillen W |
| Journal | FEMS Microbiol Lett |
| Volume | 117 |
| Issue | 2 |
| Pagination | 137-42 |
| Date Published | 1994 Apr 01 |
| ISSN | 0378-1097 |
| Keywords | Acinetobacter calcoaceticus, Alkyl and Aryl Transferases, Amino Acid Sequence, Base Sequence, DNA-Binding Proteins, DNA-Directed RNA Polymerases, Drug Resistance, Microbial, Fosfomycin, Gene Expression, Genetic Complementation Test, Molecular Sequence Data, Open Reading Frames, RNA Polymerase Sigma 54, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Sigma Factor, Transferases |
| Abstract | We have analyzed the sequence downstream of rpoN from Acinetobacter calcoaceticus and identified an open reading frame encoding a protein with high similarity to UDP-N-acetylglucosamine 1-carboxyvinyl-transferase (MurZ). Multicopy plasmids encoding this enzyme conferred phosphomycin resistance to A. calcoaceticus. The polar effect of a rpoN mutation on the phosphomycin resistance level suggests that murZ is, in part, cotranscribed with rpoN. These observations confirm that A. calcoaceticus represents the first exception from a conserved genetic context of rpoN observed in several other Gram-negative bacteria. |
| DOI | 10.1111/j.1574-6968.1994.tb06755.x |
| Alternate Journal | FEMS Microbiol Lett |
| PubMed ID | 8181716 |