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Transcription termination occurs within a 1000 base pair region downstream from the poly(A) site of the mouse beta-globin (major) gene.

TitleTranscription termination occurs within a 1000 base pair region downstream from the poly(A) site of the mouse beta-globin (major) gene.
Publication TypeJournal Article
Year of Publication1984
AuthorsCitron B, Falck-Pedersen E, Salditt-Georgieff M, Darnell JE
JournalNucleic Acids Res
Volume12
Issue22
Pagination8723-31
Date Published1984 Nov 26
ISSN0305-1048
KeywordsAnimals, Base Composition, Base Sequence, Cell Line, DNA Restriction Enzymes, Genes, Globins, Leukemia, Experimental, Mice, Nucleic Acid Hybridization, Poly A, Transcription, Genetic
Abstract

Transcription of RNA from the beta-globin (major) gene in nuclei from induced mouse erythroleukemia cells terminates within the region between 700 to 2000 bases downstream from the poly(A) addition site, but not at particularly favored sites. In addition we present the first analysis of in vivo labeled RNA from a cell transcription unit that shows RNA termination in the same region as diagnosed by analysis of in vitro labeled RNA. The region in which termination occurs is contained in 1414 bp that were sequenced beginning 600 bases downstream from the poly(A) site. There is an increased frequency of the sequence AATAAA at the beginning and a stem and loop structure followed by a string of Ts near the end of this region.

DOI10.1093/nar/12.22.8723
Alternate JournalNucleic Acids Res
PubMed ID6095210
PubMed Central IDPMC320410
Grant ListA107233-08 / / PHS HHS / United States
CA 16006-11 / CA / NCI NIH HHS / United States
CA 18213-09 / CA / NCI NIH HHS / United States

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