|Title||Topological linkage of circular DNA molecules promoted by Ustilago rec 1 protein and topoisomerase.|
|Publication Type||Journal Article|
|Year of Publication||1983|
|Authors||Kmiec EB, Kroeger PE, Brougham MJ, Holloman WK|
|Date Published||1983 Oct|
|Keywords||Adenosine Triphosphate, Adenylyl Imidodiphosphate, Basidiomycota, DNA Topoisomerases, Type I, DNA, Circular, Exodeoxyribonuclease V, Fungal Proteins, Genetic Linkage, Nucleic Acid Conformation, Ustilago|
We studied the formation of linked circular DNA molecules promoted by the combined action of rec 1 protein and type I topoisomerase of Ustilago maydis. When ATP was added as cofactor to reactions containing rec 1 protein, pairs of homologous circular DNA molecules became linked after addition of topoisomerase. Closed circular duplex molecules could be joined at homologous sites with circular single-stranded molecules or with other circular duplex molecules, provided that homologous single-stranded DNA fragments or RNA polymerase and nucleoside triphosphates were also added. Complexes formed were topologically linked through regions of heteroduplex DNA. When the analog adenylyl-imidodiphosphate was substituted for ATP, nonhomologous pairs of circular DNA molecules became linked.
|Grant List||GM 27103 / GM / NIGMS NIH HHS / United States|