|Topological linkage of circular DNA molecules promoted by Ustilago rec 1 protein and topoisomerase.
|Year of Publication
|Kmiec EB, Kroeger PE, Brougham MJ, Holloman WK
|Adenosine Triphosphate, Adenylyl Imidodiphosphate, Basidiomycota, DNA Topoisomerases, Type I, DNA, Circular, Exodeoxyribonuclease V, Fungal Proteins, Genetic Linkage, Nucleic Acid Conformation, Ustilago
We studied the formation of linked circular DNA molecules promoted by the combined action of rec 1 protein and type I topoisomerase of Ustilago maydis. When ATP was added as cofactor to reactions containing rec 1 protein, pairs of homologous circular DNA molecules became linked after addition of topoisomerase. Closed circular duplex molecules could be joined at homologous sites with circular single-stranded molecules or with other circular duplex molecules, provided that homologous single-stranded DNA fragments or RNA polymerase and nucleoside triphosphates were also added. Complexes formed were topologically linked through regions of heteroduplex DNA. When the analog adenylyl-imidodiphosphate was substituted for ATP, nonhomologous pairs of circular DNA molecules became linked.
|GM 27103 / GM / NIGMS NIH HHS / United States
Submitted by alp2017 on April 24, 2015 - 10:50am