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Telomerase core components protect Candida telomeres from aberrant overhang accumulation.

TitleTelomerase core components protect Candida telomeres from aberrant overhang accumulation.
Publication TypeJournal Article
Year of Publication2007
AuthorsHsu M, McEachern MJ, Dandjinou AT, Tzfati Y, Orr E, Blackburn EH, Lue NF
JournalProc Natl Acad Sci U S A
Volume104
Issue28
Pagination11682-7
Date Published2007 Jul 10
ISSN0027-8424
KeywordsCandida, DNA, Fungal, RNA, Telomerase, Telomere
Abstract

Telomerase is a cellular reverse transcriptase that extends one strand (the G-strand) of the telomere terminal repeats. Aside from this role in telomere length maintenance, telomerase has been proposed to serve a protective function at chromosome ends, although this is not well understood mechanistically. Earlier analysis suggests that, in the pathogenic yeast Candida albicans, the catalytic reverse transcriptase subunit of telomerase (TERT/EST2) can protect telomeres against nucleolytic degradation. In this report we demonstrate that the RNA component (TER1) has a similar function; in addition to complete loss of telomerase activity and progressive telomere attrition, the ter1-DeltaDelta strains manifested a dramatic increase in the amount of G-strand overhangs, consistent with aberrant degradation of the complementary C-strand. We also demonstrate that a catalytically incompetent EST2 protein can suppress such overhang accumulation in the est2-DeltaDelta mutant to the same extent as the wild-type protein. Altogether, our data support the notion that the Candida telomerase core components mediate a protective function through a mechanism that is independent of its catalytic activity.

DOI10.1073/pnas.0700327104
Alternate JournalProc Natl Acad Sci U S A
PubMed ID17609387
PubMed Central IDPMC1913905
Grant ListGM069507 / GM / NIGMS NIH HHS / United States
GM 26259 / GM / NIGMS NIH HHS / United States
DE11356 / DE / NIDCR NIH HHS / United States
R01 GM069507 / GM / NIGMS NIH HHS / United States
R01 GM026259 / GM / NIGMS NIH HHS / United States
R37 GM026259 / GM / NIGMS NIH HHS / United States

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