Submitted by alp2017 on April 24, 2015 - 10:49am
Title | Synapsis promoted by Ustilago rec1 protein. |
Publication Type | Journal Article |
Year of Publication | 1984 |
Authors | Kmiec EB, Holloman WK |
Journal | Cell |
Volume | 36 |
Issue | 3 |
Pagination | 593-8 |
Date Published | 1984 Mar |
ISSN | 0092-8674 |
Keywords | Adenosine Diphosphate, DNA Helicases, Exodeoxyribonuclease V, Fungal Proteins, Meiosis, Nucleic Acid Conformation, Recombination, Genetic, Ustilago |
Abstract | Ustilago rec 1 protein pairs homologous DNA molecules by promoting both synapsis and strand transfer. Complexes formed with rec 1 protein and a homologous combination of single-stranded and duplex DNA that appear to be synaptic structures can be detected by use of a nitrocellulose filter assay. The nascent heteroduplex formed during synapsis is a paranemic joint in which the single-stranded DNA pairs, but does not interwind, with its complement in the duplex molecule. Formation of the paranemic joint is accompanied by duplex unwinding and genesis of left-handed Z-DNA. |
Alternate Journal | Cell |
PubMed ID | 6321036 |
Grant List | GM 27103 / GM / NIGMS NIH HHS / United States |