Synapsis promoted by Ustilago rec1 protein.

TitleSynapsis promoted by Ustilago rec1 protein.
Publication TypeJournal Article
Year of Publication1984
AuthorsKmiec EB, Holloman WK
Date Published1984 Mar
KeywordsAdenosine Diphosphate, DNA Helicases, Exodeoxyribonuclease V, Fungal Proteins, Meiosis, Nucleic Acid Conformation, Recombination, Genetic, Ustilago

Ustilago rec 1 protein pairs homologous DNA molecules by promoting both synapsis and strand transfer. Complexes formed with rec 1 protein and a homologous combination of single-stranded and duplex DNA that appear to be synaptic structures can be detected by use of a nitrocellulose filter assay. The nascent heteroduplex formed during synapsis is a paranemic joint in which the single-stranded DNA pairs, but does not interwind, with its complement in the duplex molecule. Formation of the paranemic joint is accompanied by duplex unwinding and genesis of left-handed Z-DNA.

Alternate JournalCell
PubMed ID6321036
Grant ListGM 27103 / GM / NIGMS NIH HHS / United States

Weill Cornell Medicine Microbiology and Immunology 1300 York Avenue, Box 62 New York, NY 10065 Phone: (212) 746-6505 Fax: (212) 746-8587