Synapsis promoted by Ustilago rec1 protein.

TitleSynapsis promoted by Ustilago rec1 protein.
Publication TypeJournal Article
Year of Publication1984
AuthorsKmiec EB, Holloman WK
JournalCell
Volume36
Issue3
Pagination593-8
Date Published1984 Mar
ISSN0092-8674
KeywordsAdenosine Diphosphate, DNA Helicases, Exodeoxyribonuclease V, Fungal Proteins, Meiosis, Nucleic Acid Conformation, Recombination, Genetic, Ustilago
Abstract

Ustilago rec 1 protein pairs homologous DNA molecules by promoting both synapsis and strand transfer. Complexes formed with rec 1 protein and a homologous combination of single-stranded and duplex DNA that appear to be synaptic structures can be detected by use of a nitrocellulose filter assay. The nascent heteroduplex formed during synapsis is a paranemic joint in which the single-stranded DNA pairs, but does not interwind, with its complement in the duplex molecule. Formation of the paranemic joint is accompanied by duplex unwinding and genesis of left-handed Z-DNA.

Alternate JournalCell
PubMed ID6321036
Grant ListGM 27103 / GM / NIGMS NIH HHS / United States

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