Title | Structure of REC2, a recombinational repair gene of Ustilago maydis, and its function in homologous recombination between plasmid and chromosomal sequences. |
Publication Type | Journal Article |
Year of Publication | 1994 |
Authors | Rubin BP, Ferguson DO, Holloman WK |
Journal | Mol Cell Biol |
Volume | 14 |
Issue | 9 |
Pagination | 6287-96 |
Date Published | 1994 Sep |
ISSN | 0270-7306 |
Keywords | Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA Primers, DNA Repair, DNA, Fungal, Fungal Proteins, Genes, Fungal, Molecular Sequence Data, Mutagenesis, Site-Directed, Recombination, Genetic, Restriction Mapping, Sequence Alignment, Structure-Activity Relationship, Ustilago |
Abstract | Mutation in the REC2 gene of Ustilago maydis leads to defects in DNA repair, recombination, and meiosis. Analysis of the primary sequence of the Rec2 protein reveals a region with significant homology to bacterial RecA protein and to the yeast recombination proteins Dmc1, Rad51, and Rad57. This homologous region in the U. maydis Rec2 protein was found to be functionally sensitive to mutation, lending support to the hypothesis that Rec2 has a functional RecA-like domain essential for activity in recombination and repair. Homologous recombination between plasmid and chromosomal DNA sequences is reduced substantially in the rec2 mutant following transformation. The frequency can be restored to a level approaching, but not exceeding, that observed in the wild-type strain if transformation is performed with cells containing multiple copies of REC2. |
Alternate Journal | Mol. Cell. Biol. |
PubMed ID | 8065360 |
PubMed Central ID | PMC359155 |
Grant List | GM42482 / GM / NIGMS NIH HHS / United States |
Submitted by alp2017 on April 24, 2015 - 10:43am