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Structure of REC2, a recombinational repair gene of Ustilago maydis, and its function in homologous recombination between plasmid and chromosomal sequences.

TitleStructure of REC2, a recombinational repair gene of Ustilago maydis, and its function in homologous recombination between plasmid and chromosomal sequences.
Publication TypeJournal Article
Year of Publication1994
AuthorsRubin BP, Ferguson DO, Holloman WK
JournalMol Cell Biol
Volume14
Issue9
Pagination6287-96
Date Published1994 Sep
ISSN0270-7306
KeywordsAmino Acid Sequence, Base Sequence, Cloning, Molecular, DNA Primers, DNA Repair, DNA, Fungal, Fungal Proteins, Genes, Fungal, Molecular Sequence Data, Mutagenesis, Site-Directed, Recombination, Genetic, Restriction Mapping, Sequence Alignment, Structure-Activity Relationship, Ustilago
Abstract

Mutation in the REC2 gene of Ustilago maydis leads to defects in DNA repair, recombination, and meiosis. Analysis of the primary sequence of the Rec2 protein reveals a region with significant homology to bacterial RecA protein and to the yeast recombination proteins Dmc1, Rad51, and Rad57. This homologous region in the U. maydis Rec2 protein was found to be functionally sensitive to mutation, lending support to the hypothesis that Rec2 has a functional RecA-like domain essential for activity in recombination and repair. Homologous recombination between plasmid and chromosomal DNA sequences is reduced substantially in the rec2 mutant following transformation. The frequency can be restored to a level approaching, but not exceeding, that observed in the wild-type strain if transformation is performed with cells containing multiple copies of REC2.

Alternate JournalMol. Cell. Biol.
PubMed ID8065360
PubMed Central IDPMC359155
Grant ListGM42482 / GM / NIGMS NIH HHS / United States

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