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Statistical positioning of nucleosomes by specific protein-binding to an upstream activating sequence in yeast.

TitleStatistical positioning of nucleosomes by specific protein-binding to an upstream activating sequence in yeast.
Publication TypeJournal Article
Year of Publication1988
AuthorsFedor MJ, Lue NF, Kornberg RD
JournalJ Mol Biol
Volume204
Issue1
Pagination109-27
Date Published1988 Nov 05
ISSN0022-2836
KeywordsBase Sequence, Binding Sites, Chromatin, Chromosomes, DNA, Fungal, Fungal Proteins, Nucleosomes, Regulatory Sequences, Nucleic Acid, Saccharomyces cerevisiae
Abstract

Arrays of nucleosomes were positioned with respect to the GAL1-GAL10 intergenic region inserted into Saccharomyces cerevisiae minichromosomes. Deletions of DNA flanking the upstream activation sequence left the array unaltered, showing that nucleosome positioning was not a consequence of sequence-specific histone-DNA interactions but depended on proximity to the galactose-responsive upstream activation sequence (UASG). Replacement of the upstream activation sequence by synthetic oligonucleotides with different protein-binding properties identified a short sequence within this region that is responsible for the ordered array. This sequence overlaps a binding site for GAL4 protein, a positive regulator of transcription, but exerts its effect on chromatin structure independently of GAL4, probably through binding a novel factor that is not GAL-specific.

DOI10.1016/0022-2836(88)90603-1
Alternate JournalJ Mol Biol
PubMed ID3063825

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