Title | Statistical positioning of nucleosomes by specific protein-binding to an upstream activating sequence in yeast. |
Publication Type | Journal Article |
Year of Publication | 1988 |
Authors | Fedor MJ, Lue NF, Kornberg RD |
Journal | J Mol Biol |
Volume | 204 |
Issue | 1 |
Pagination | 109-27 |
Date Published | 1988 Nov 05 |
ISSN | 0022-2836 |
Keywords | Base Sequence, Binding Sites, Chromatin, Chromosomes, DNA, Fungal, Fungal Proteins, Nucleosomes, Regulatory Sequences, Nucleic Acid, Saccharomyces cerevisiae |
Abstract | Arrays of nucleosomes were positioned with respect to the GAL1-GAL10 intergenic region inserted into Saccharomyces cerevisiae minichromosomes. Deletions of DNA flanking the upstream activation sequence left the array unaltered, showing that nucleosome positioning was not a consequence of sequence-specific histone-DNA interactions but depended on proximity to the galactose-responsive upstream activation sequence (UASG). Replacement of the upstream activation sequence by synthetic oligonucleotides with different protein-binding properties identified a short sequence within this region that is responsible for the ordered array. This sequence overlaps a binding site for GAL4 protein, a positive regulator of transcription, but exerts its effect on chromatin structure independently of GAL4, probably through binding a novel factor that is not GAL-specific. |
DOI | 10.1016/0022-2836(88)90603-1 |
Alternate Journal | J Mol Biol |
PubMed ID | 3063825 |
Submitted by jom4013 on December 3, 2020 - 4:18pm