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Species-specific and sequence-specific recognition of the dG-rich strand of telomeres by yeast telomerase.

TitleSpecies-specific and sequence-specific recognition of the dG-rich strand of telomeres by yeast telomerase.
Publication TypeJournal Article
Year of Publication1998
AuthorsLue NF, Xia J
JournalNucleic Acids Res
Volume26
Issue6
Pagination1495-502
Date Published1998 Mar 15
ISSN0305-1048
KeywordsAnimals, Base Composition, Base Sequence, Binding Sites, Humans, In Vitro Techniques, Kinetics, Protein Conformation, Saccharomyces cerevisiae, Species Specificity, Substrate Specificity, Telomerase, Telomere, Tetrahymena
Abstract

A gel mobility shift assay was developed to examine recognition of yeast telomeres by telomerase. An RNase-sensitive G-rich strand-specific binding activity can be detected in partially purified yeast telomerase fractions. The binding activity was attributed to telomerase, because it co-purifies with TLC1 RNA and telomerase activity over three different chromatographic steps and because the complex co-migrates with TLC1 RNA when subjected to electrophoresis through native gels. Analysis of the binding specificity of yeast telomerase indicates that it recognizes the G-rich strand of yeast telomeres with high affinity and specificity. The K d for the interaction is approximately 3 nM. Single-stranded G-rich telomeres from other species, such as human and Tetrahymena, though capable of being extended by yeast telomerase in polymerization assays at high concentrations, bind the enzyme with at least 100-fold lower affinities. The ability of a sequence to be bound tightly by yeast telomerase in vitro correlates with its ability to seed telomere formation in vivo. The implications of these findings for regulation of telomerase activity are discussed.

DOI10.1093/nar/26.6.1495
Alternate JournalNucleic Acids Res
PubMed ID9490797
PubMed Central IDPMC147437

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