Submitted by jom4013 on December 3, 2020 - 4:11pm
| Title | Soluble CD8 attenuates cytotoxic T cell responses against replication-defective adenovirus affording transprotection of transgenes in vivo. |
| Publication Type | Journal Article |
| Year of Publication | 2000 |
| Authors | Peng Y, Falck-Pedersen E, Elkon KB |
| Journal | J Immunol |
| Volume | 165 |
| Issue | 3 |
| Pagination | 1470-8 |
| Date Published | 2000 Aug 01 |
| ISSN | 0022-1767 |
| Keywords | Adenoviridae, Animals, CD8 Antigens, CD8-Positive T-Lymphocytes, Cytotoxicity Tests, Immunologic, Cytotoxicity, Immunologic, Defective Viruses, Epitopes, T-Lymphocyte, Gene Expression Regulation, Genetic Vectors, Immunosuppressive Agents, Injections, Intravenous, Liver, Lymphocyte Activation, Mice, Mice, Inbred BALB C, Mice, Inbred C3H, Mice, Inbred C57BL, Mice, Transgenic, Ovalbumin, Recombinant Fusion Proteins, Solubility, T-Lymphocytes, Cytotoxic, Transgenes, Virus Replication |
| Abstract | The T cell coreceptor, CD8, enhances T cell-APC interactions. Because soluble CD8alpha homodimers can antagonize CD8 T cell activation in vitro, we asked whether secretion of soluble CD8 would effect cytotoxic T cell responses in vivo. Production of soluble CD8 by a replication-defective adenovirus vector allowed persistent virus expression for up to 5 mo in C57BL/6 mice and protected a second foreign transgene from rapid deletion. Soluble CD8 selectively inhibited CD8 T cell proliferation and IFN-gamma production and could also attenuate peptide-specific CD8 T cell responses in vivo. These finding suggest that gene vector delivery of soluble CD8 may have therapeutic applications. |
| DOI | 10.4049/jimmunol.165.3.1470 |
| Alternate Journal | J Immunol |
| PubMed ID | 10903752 |
| Grant List | HL-9308-L / HL / NHLBI NIH HHS / United States |