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Second-end capture in DNA double-strand break repair promoted by Brh2 protein of Ustilago maydis.

TitleSecond-end capture in DNA double-strand break repair promoted by Brh2 protein of Ustilago maydis.
Publication TypeJournal Article
Year of Publication2009
AuthorsMazloum N, Holloman WK
JournalMol Cell
Volume33
Issue2
Pagination160-70
Date Published2009 Jan 30
ISSN1097-4164
KeywordsDNA Breaks, Double-Stranded, DNA Repair, DNA, Fungal, DNA, Single-Stranded, Fungal Proteins, Models, Genetic, Recombination, Genetic, Ustilago
Abstract

Brh2 plays a central role in the homologous recombination system of Ustilago maydis, mediating delivery of Rad51 to single-stranded DNA. Here we report that Brh2 can pair the displaced strand of a D loop with a complementary single-stranded DNA to form a duplexed, or double, D loop. The reaction emulates the second-end capture step envisioned in models of DNA double-strand break repair. This second-end capture reaction promoted by Brh2 proceeds efficiently when performed in the presence of Rad51 under conditions that block annealing by Rad52, or when the second single-stranded DNA substrate is replaced by double-stranded DNA. In a coupled reaction that requires extension of the D loop more than 200 nt by DNA synthesis in order to reveal a complementary region, Brh2 was also able to promote second-end capture and thus model a synthesis-dependent strand-annealing mechanism.

DOI10.1016/j.molcel.2008.12.023
Alternate JournalMol. Cell
PubMed ID19187759
PubMed Central IDPMC2663533
Grant ListGM42482 / GM / NIGMS NIH HHS / United States
GM79859 / GM / NIGMS NIH HHS / United States
R01 GM042482-18 / GM / NIGMS NIH HHS / United States
R01 GM079859-02 / GM / NIGMS NIH HHS / United States

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