Submitted by jom4013 on December 3, 2020 - 4:18pm
| Title | Resolution of factors required for the initiation of transcription by yeast RNA polymerase II. |
| Publication Type | Journal Article |
| Year of Publication | 1990 |
| Authors | Flanagan PM, Kelleher RJ, Feaver WJ, Lue NF, LaPointe JW, Kornberg RD |
| Journal | J Biol Chem |
| Volume | 265 |
| Issue | 19 |
| Pagination | 11105-7 |
| Date Published | 1990 Jul 05 |
| ISSN | 0021-9258 |
| Keywords | Cell Nucleus, Escherichia coli, HeLa Cells, RNA Polymerase II, Saccharomyces cerevisiae, Transcription Factor TFIIA, Transcription Factor TFIID, Transcription Factors, Transcription, Genetic |
| Abstract | Fractionation of a yeast nuclear extract reveals at least four factors required in addition to RNA polymerase II for accurate initiation of transcription. One of these factors can be replaced by HeLa transcription factor IID or by its yeast counterpart expressed in Escherichia coli. Each of the remaining three factors can be replaced by a fraction from yeast whole cell extract, facilitating further purification of the factors. |
| Alternate Journal | J Biol Chem |
| PubMed ID | 2193032 |
| Grant List | GM-07365 / GM / NIGMS NIH HHS / United States GM-36659 / GM / NIGMS NIH HHS / United States |