For COVID-19 vaccine updates, please review our information guide. For patient eligibility and scheduling availability, please visit VaccineTogetherNY.org.

Resolution of factors required for the initiation of transcription by yeast RNA polymerase II.

TitleResolution of factors required for the initiation of transcription by yeast RNA polymerase II.
Publication TypeJournal Article
Year of Publication1990
AuthorsFlanagan PM, Kelleher RJ, Feaver WJ, Lue NF, LaPointe JW, Kornberg RD
JournalJ Biol Chem
Volume265
Issue19
Pagination11105-7
Date Published1990 Jul 05
ISSN0021-9258
KeywordsCell Nucleus, Escherichia coli, HeLa Cells, RNA Polymerase II, Saccharomyces cerevisiae, Transcription Factor TFIIA, Transcription Factor TFIID, Transcription Factors, Transcription, Genetic
Abstract

Fractionation of a yeast nuclear extract reveals at least four factors required in addition to RNA polymerase II for accurate initiation of transcription. One of these factors can be replaced by HeLa transcription factor IID or by its yeast counterpart expressed in Escherichia coli. Each of the remaining three factors can be replaced by a fraction from yeast whole cell extract, facilitating further purification of the factors.

Alternate JournalJ Biol Chem
PubMed ID2193032
Grant ListGM-07365 / GM / NIGMS NIH HHS / United States
GM-36659 / GM / NIGMS NIH HHS / United States

Weill Cornell Medicine Microbiology and Immunology 1300 York Avenue, Box 62 New York, NY 10065 Phone: (212) 746-6505 Fax: (212) 746-8587