For COVID-19 vaccine updates, please review our information guide. For patient eligibility and scheduling availability, please visit VaccineTogetherNY.org.

Regulation of telomere structure and functions by subunits of the INO80 chromatin remodeling complex.

TitleRegulation of telomere structure and functions by subunits of the INO80 chromatin remodeling complex.
Publication TypeJournal Article
Year of Publication2007
AuthorsYu EYoung, Steinberg-Neifach O, Dandjinou AT, Kang F, Morrison AJ, Shen X, Lue NF
JournalMol Cell Biol
Volume27
Issue16
Pagination5639-49
Date Published2007 Aug
ISSN0270-7306
KeywordsChromatin Assembly and Disassembly, Microfilament Proteins, Mutation, Protein Binding, Protein Structure, Tertiary, Protein Subunits, Protein Transport, Saccharomyces cerevisiae Proteins, Telomerase, Telomere
Abstract

ATP-dependent chromatin remodeling complexes have been implicated in the regulation of transcription, replication, and more recently DNA double-strand break repair. Here we report that the Ies3p subunit of the Saccharomyces cerevisiae INO80 chromatin remodeling complex interacts with a conserved tetratricopeptide repeat domain of the telomerase protein Est1p. Deletion of IES3 and some other subunits of the complex induced telomere elongation and altered telomere position effect. In telomerase-negative mutants, loss of Ies3p delayed the emergence of recombinational survivors and stimulated the formation of extrachromosomal telomeric circles in survivors. Deletion of IES3 also resulted in heightened levels of telomere-telomere fusions in telomerase-deficient strains. In addition, a delay in survivor formation was observed in an Arp8p-deficient mutant. Because Arp8p is required for the chromatin remodeling activity of the INO80 complex, the complex may promote recombinational telomere maintenance by altering chromatin structure. Consistent with this notion, we observed preferential localization of multiple subunits of the INO80 complex to telomeres. Our results reveal novel functions for a subunit of the telomerase complex and the INO80 chromatin remodeling complex.

DOI10.1128/MCB.00418-07
Alternate JournalMol Cell Biol
PubMed ID17562861
PubMed Central IDPMC1952117
Grant ListR01 GM062631 / GM / NIGMS NIH HHS / United States
K22 CA100017 / CA / NCI NIH HHS / United States
GM62631 / GM / NIGMS NIH HHS / United States
P30 ES007784 / ES / NIEHS NIH HHS / United States
ES07784 / ES / NIEHS NIH HHS / United States

Weill Cornell Medicine Microbiology and Immunology 1300 York Avenue, Box 62 New York, NY 10065 Phone: (212) 746-6505 Fax: (212) 746-8587