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Regulation of antibody secretion by hybridoma cells. II. Mechanism of idiotype-induced suppression of antibody secretion by hybridoma cells.

TitleRegulation of antibody secretion by hybridoma cells. II. Mechanism of idiotype-induced suppression of antibody secretion by hybridoma cells.
Publication TypeJournal Article
Year of Publication1989
AuthorsGulati P, Falck-Pedersen E, Siskind GW
JournalCell Immunol
Volume123
Issue2
Pagination276-82
Date Published1989 Oct 15
ISSN0008-8749
KeywordsAnimals, Antibodies, Monoclonal, Gene Expression Regulation, Hybridomas, Immune Tolerance, Immunoglobulin Heavy Chains, Immunoglobulin Idiotypes, Immunoglobulin Light Chains, Mice, Mice, Inbred Strains, RNA, Messenger, T-Lymphocytes, Helper-Inducer, T-Lymphocytes, Regulatory, Transcription, Genetic
Abstract

We have previously reported that antibody secretion by B.22 hybridoma cells can be suppressed in an MHC-restricted manner, by idiotype-specific T cells. It was shown that T cells of both helper and suppressor phenotypes are involved, and that the suppression is mediated by soluble factors. In the present paper, we have characterized the effects of T-cell-mediated suppression at the level of B.22 antibody mRNA expression and stability. Nuclear run-on analysis comparing suppressed and control B.22 cells indicates no change in the transcription rates of heavy and light chains. Northern blot analysis demonstrates that steady-state levels of heavy and light chain mRNAs are also unchanged. Thus, the suppression of antibody secretion by B.22 cells probably occurs at the levels of translation or secretion.

DOI10.1016/0008-8749(89)90288-8
Alternate JournalCell Immunol
PubMed ID2529040
Grant ListAI 11694 / AI / NIAID NIH HHS / United States

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