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Regulated Expression Systems for Mycobacteria and Their Applications.

TitleRegulated Expression Systems for Mycobacteria and Their Applications.
Publication TypeJournal Article
Year of Publication2014
AuthorsSchnappinger D, Ehrt S
JournalMicrobiol Spectr
Date Published2014

For bacterial model organisms like and genetic tools to experimentally manipulate the activity of individual genes existed for decades. But for genetically less tractable yet medically important bacteria such as such tools have rarely been available. More recently several groups developed genetic switches that function efficiently in and other mycobacteria. Together these systems utilize six different transcription factors, eight different regulated promoters, and three different regulatory principles. Here we describe their design features, review their main applications, and discuss advantages and disadvantages of regulating transcription, translation, or protein stability for controlling gene activities in bacteria.

Alternate JournalMicrobiol Spectr
PubMed ID25485177
PubMed Central IDPMC4254785
Grant ListR01 AI063446 / AI / NIAID NIH HHS / United States
R01 AI091790 / AI / NIAID NIH HHS / United States
R01 AI092573 / AI / NIAID NIH HHS / United States

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