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Purification and properties of a cruciform DNA binding protein from Ustilago maydis.

TitlePurification and properties of a cruciform DNA binding protein from Ustilago maydis.
Publication TypeJournal Article
Year of Publication1993
AuthorsKotani H, Kmiec EB, Holloman WK
JournalChromosoma
Volume102
Issue5
Pagination348-54
Date Published1993 May
ISSN0009-5915
KeywordsBase Sequence, Chromatography, Gel, DNA, Fungal, DNA-Binding Proteins, Kinetics, Molecular Sequence Data, Nucleic Acid Conformation, Protein Binding, Solubility, Ustilago
Abstract

A DNA binding protein with an M(r) of 11,000 was purified from Ustilago maydis. Its solubility in acid, amino acid composition, and mobility during gel electrophoresis are reminiscent of properties observed for the high mobility group nonhistone chromosomal proteins. The protein recognizes cruciform DNA made from oligonucleotides and also binds preferentially to a plasmid containing an extruded cruciform.

Alternate JournalChromosoma
PubMed ID8325166

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