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A possible role for the yeast TATA-element-binding protein in DNA replication.

TitleA possible role for the yeast TATA-element-binding protein in DNA replication.
Publication TypeJournal Article
Year of Publication1993
AuthorsLue NF, Kornberg RD
JournalProc Natl Acad Sci U S A
Volume90
Issue17
Pagination8018-22
Date Published1993 Sep 01
ISSN0027-8424
KeywordsBase Sequence, Binding Sites, DNA Replication, DNA, Fungal, DNA-Binding Proteins, Mitosis, Molecular Sequence Data, Mutagenesis, Insertional, Oligodeoxyribonucleotides, Restriction Mapping, Saccharomyces cerevisiae, TATA Box, TATA-Box Binding Protein, Transcription Factors
Abstract

The TATA-element-binding protein (TBP) is involved in the initiation of transcription by all three eukaryotic RNA polymerases. The following observations implicate TBP in the initiation of DNA replication at yeast chromosomal origins as well: (i) Recombinant yeast TBP binds specifically to functionally important regions of many yeast replication origins in vitro. (ii) TBP-binding sites from RNA polymerase II promoters can activate defective replication origins in vivo. (iii) Point mutations in TBP-binding sites that diminish their affinity for TBP in vitro reduce their ability to support replication in vivo.

DOI10.1073/pnas.90.17.8018
Alternate JournalProc Natl Acad Sci U S A
PubMed ID8367459
PubMed Central IDPMC47279

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