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A poly(A) addition site and a downstream termination region are required for efficient cessation of transcription by RNA polymerase II in the mouse beta maj-globin gene.

TitleA poly(A) addition site and a downstream termination region are required for efficient cessation of transcription by RNA polymerase II in the mouse beta maj-globin gene.
Publication TypeJournal Article
Year of Publication1987
AuthorsLogan J, Falck-Pedersen E, Darnell JE, Shenk T
JournalProc Natl Acad Sci U S A
Volume84
Issue23
Pagination8306-10
Date Published1987 Dec
ISSN0027-8424
KeywordsAnimals, DNA Mutational Analysis, Genes, Regulator, Globins, Mice, Poly A, RNA Polymerase II, RNA, Messenger, Terminator Regions, Genetic, Transcription, Genetic
Abstract

Sequence elements within the mouse beta maj-globin transcription unit required for efficient termination of transcription by RNA polymerase II have been delineated. To facilitate nascent-chain analysis of termination, the DNA segment in which transcription ceases was introduced into the adenovirus chromosome within its E1A transcription unit. Two beta-globin DNA elements were required to effect efficient termination: an upstream sequence that includes two poly(A) addition signals and a downstream region previously shown to be where RNA synthesis stops. The role of poly(A) addition in termination was established by introduction of several single base pair substitutions into the AATAAA polyadenylylation motifs. These mutations inhibited both polyadenylylation and termination within the beta-globin DNA segment. Therefore, poly(A) addition appears to be a prerequisite for efficient termination.

DOI10.1073/pnas.84.23.8306
Alternate JournalProc Natl Acad Sci U S A
PubMed ID3479794
PubMed Central IDPMC299531
Grant ListCA-38965 / CA / NCI NIH HHS / United States

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