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Persistent Mycobacterium tuberculosis infection in mice requires PerM for successful cell division.

TitlePersistent Mycobacterium tuberculosis infection in mice requires PerM for successful cell division.
Publication TypeJournal Article
Year of Publication2019
AuthorsWang R, Kreutzfeldt K, Botella H, Vaubourgeix J, Schnappinger D, Ehrt S
JournalElife
Volume8
Date Published2019 11 21
ISSN2050-084X
KeywordsAnimals, Bacterial Proteins, Cell Cycle Proteins, Cell Division, Disease Models, Animal, Female, Gene Expression Regulation, Bacterial, Gene Knockout Techniques, Lung, Membrane Proteins, Mice, Mice, Inbred C57BL, Mycobacterium tuberculosis, Phenotype, Tuberculosis
Abstract

The ability of Mycobacterium tuberculosis (Mtb) to persist in its host is central to the pathogenesis of tuberculosis, yet the underlying mechanisms remain incompletely defined. PerM, an integral membrane protein, is required for persistence of Mtb in mice. Here, we show that deletion caused a cell division defect specifically during the chronic phase of mouse infection, but did not affect Mtb's cell replication during acute infection. We further demonstrate that PerM is required for cell division in chronically infected mice and in vitro under host-relevant stresses because it is part of the mycobacterial divisome and stabilizes the essential divisome protein FtsB. These data highlight the importance of sustained cell division for Mtb persistence, define condition-specific requirements for cell division and reveal that survival of Mtb during chronic infection depends on a persistence divisome.

DOI10.7554/eLife.49570
Alternate JournalElife
PubMed ID31751212
PubMed Central IDPMC6872210
Grant ListU19 AI111143 / AI / NIAID NIH HHS / United States

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