Title | Pathways of transformation in Ustilago maydis determined by DNA conformation. |
Publication Type | Journal Article |
Year of Publication | 1990 |
Authors | Fotheringham S, Holloman WK |
Journal | Genetics |
Volume | 124 |
Issue | 4 |
Pagination | 833-43 |
Date Published | 1990 Apr |
ISSN | 0016-6731 |
Keywords | Basidiomycota, Blotting, Southern, Cloning, Molecular, DNA Replication, DNA, Circular, DNA, Fungal, DNA, Recombinant, Nucleic Acid Conformation, Plasmids, Repetitive Sequences, Nucleic Acid, Transformation, Genetic, Ustilago |
Abstract | Ustilago maydis was transformed by plasmids bearing a cloned, selectable gene but lacking an autonomously replicating sequence. Transformation was primarily through integration at nonhomologous loci when the plasmid DNA was circular. When the DNA was made linear by cleavage within the cloned gene, the spectrum of integration events shifted from random to targeted recombination at the resident chromosomal allele. In a large fraction of the transformants obtained using linear DNA, the plasmid DNA was not integrated but was maintained in an extrachromosomal state composed of a concatameric array of plasmid units joined end-to-end. The results suggest the operation of several pathways for transformation in U. maydis, and that DNA conformation at the time of transformation governs choice of pathways. |
Alternate Journal | Genetics |
PubMed ID | 2323553 |
PubMed Central ID | PMC1203975 |
Grant List | GM42548 / GM / NIGMS NIH HHS / United States |
Submitted by alp2017 on April 24, 2015 - 10:46am