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Pathways of transformation in Ustilago maydis determined by DNA conformation.

TitlePathways of transformation in Ustilago maydis determined by DNA conformation.
Publication TypeJournal Article
Year of Publication1990
AuthorsFotheringham S, Holloman WK
JournalGenetics
Volume124
Issue4
Pagination833-43
Date Published1990 Apr
ISSN0016-6731
KeywordsBasidiomycota, Blotting, Southern, Cloning, Molecular, DNA Replication, DNA, Circular, DNA, Fungal, DNA, Recombinant, Nucleic Acid Conformation, Plasmids, Repetitive Sequences, Nucleic Acid, Transformation, Genetic, Ustilago
Abstract

Ustilago maydis was transformed by plasmids bearing a cloned, selectable gene but lacking an autonomously replicating sequence. Transformation was primarily through integration at nonhomologous loci when the plasmid DNA was circular. When the DNA was made linear by cleavage within the cloned gene, the spectrum of integration events shifted from random to targeted recombination at the resident chromosomal allele. In a large fraction of the transformants obtained using linear DNA, the plasmid DNA was not integrated but was maintained in an extrachromosomal state composed of a concatameric array of plasmid units joined end-to-end. The results suggest the operation of several pathways for transformation in U. maydis, and that DNA conformation at the time of transformation governs choice of pathways.

Alternate JournalGenetics
PubMed ID2323553
PubMed Central IDPMC1203975
Grant ListGM42548 / GM / NIGMS NIH HHS / United States

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