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Ortholog of BRCA2-interacting protein BCCIP controls morphogenetic responses during DNA replication stress in Ustilago maydis.

TitleOrtholog of BRCA2-interacting protein BCCIP controls morphogenetic responses during DNA replication stress in Ustilago maydis.
Publication TypeJournal Article
Year of Publication2007
AuthorsMao N, Zhou Q, Kojic M, Pérez-Martín J, Holloman WK
JournalDNA Repair (Amst)
Volume6
Issue11
Pagination1651-60
Date Published2007 Nov
ISSN1568-7864
KeywordsAmino Acid Sequence, DNA Replication, Fungal Proteins, Molecular Sequence Data, Morphogenesis, Recombination, Genetic, Sequence Alignment, Ustilago
Abstract

The BRCA2 tumor suppressor functions in repair of DNA by homologous recombination through regulating the action of Rad51. In turn, BRCA2 appears to be regulated by other interacting proteins. Dss1, a small interacting protein that binds to the C-terminal domain, has a profound effect on activity as deduced from studies on the BRCA2-related protein Brh2 in Ustilago maydis. Evidence accumulating in mammalian systems suggests that BCCIP, another small interacting protein that binds to the C-terminal domain of BRCA2, also serves to regulate homologous recombination activity. Here we were interested in testing the role of the putative U. maydis BCCIP ortholog Bcp1 in DNA repair and recombination. In keeping with the mammalian paradigm, Bcp1 bound to the C-terminal region of Brh2. Mutants deleted of the gene were extremely slow growing, showed a delay passing through S phase and exhibited sensitivity to hydroxyurea, but were otherwise normal in DNA repair and homologous recombination. In the absence of Bcp1 cells were unable to maintain the wild type morphology when challenged by a DNA replication stress. These results suggest that Bcp1 could be involved in coordinating morphogenetic events with DNA processing during replication.

DOI10.1016/j.dnarep.2007.05.012
Alternate JournalDNA Repair (Amst.)
PubMed ID17627904
PubMed Central IDPMC2696116
Grant ListGM42482 / GM / NIGMS NIH HHS / United States
R01 GM042482 / GM / NIGMS NIH HHS / United States
R01 GM042482-16 / GM / NIGMS NIH HHS / United States

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