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A novel antioxidant gene from Mycobacterium tuberculosis.

TitleA novel antioxidant gene from Mycobacterium tuberculosis.
Publication TypeJournal Article
Year of Publication1997
AuthorsEhrt S, Shiloh MU, Ruan J, Choi M, Gunzburg S, Nathan C, Xie Q, Riley LW
JournalJ Exp Med
Volume186
Issue11
Pagination1885-96
Date Published1997 Dec 01
ISSN0022-1007
KeywordsAmino Acid Sequence, Animals, Antioxidants, Bacterial Proteins, Base Sequence, Cloning, Molecular, DNA, Bacterial, Escherichia coli, Genes, Bacterial, Hydrogen Peroxide, Macrophages, Mice, Mice, Inbred C57BL, Molecular Sequence Data, Mycobacterium, Mycobacterium tuberculosis, Nitrites, Oxidation-Reduction, Reactive Oxygen Species, Recombinant Fusion Proteins, Transfection, Tuberculosis
Abstract

Among the major antimicrobial products of macrophages are reactive intermediates of the oxidation of nitrogen (RNI) and the reduction of oxygen (ROI). Selection of recombinants in acidified nitrite led to the cloning of a novel gene, noxR1, from a pathogenic clinical isolate of Mycobacterium tuberculosis. Expression of noxR1 conferred upon Escherichia coli and Mycobacterium smegmatis enhanced ability to resist RNI and ROI, whether the bacteria were exposed to exogenous compounds in medium or to endogenous products in macrophages. These studies provide the first identification of an RNI resistance mechanism in mycobacteria, point to a new mechanism for resistance to ROI, and raise the possibility that inhibition of the noxR1 pathway might enhance the ability of macrophages to control tuberculosis.

DOI10.1084/jem.186.11.1885
Alternate JournalJ Exp Med
PubMed ID9382887
PubMed Central IDPMC2199150
Grant ListGM-07739 / GM / NIGMS NIH HHS / United States
HL-51967 / HL / NHLBI NIH HHS / United States

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