Molecular cloning of a gene required for DNA replication in Ustilago maydis.

TitleMolecular cloning of a gene required for DNA replication in Ustilago maydis.
Publication TypeJournal Article
Year of Publication1997
AuthorsOnel K, Holloman WK
JournalMol Gen Genet
Date Published1997 Jan 27
KeywordsAmino Acid Sequence, Base Sequence, Cell Cycle Proteins, Cloning, Molecular, DNA Replication, DNA, Fungal, Fungal Proteins, G1 Phase, Gene Expression Regulation, Fungal, Genes, Fungal, Genetic Complementation Test, Molecular Sequence Data, Open Reading Frames, RNA, Fungal, RNA, Messenger, S Phase, Sequence Analysis, DNA, Ustilago

TSD2, a gene necessary for DNA synthesis in Ustilago maydis, was cloned by complementation of the temperature sensitive growth defect of a mutant known previously as pol1-1 and renamed here tsd2-1. Linkage analysis established that the cloned fragment contained an allele of tsd2-1 and not a suppressor. DNA sequence determination of the cloned DNA fragment indicated the presence of a single large uninterrupted open reading frame capable of encoding a protein of 845 amino acids without homology to any known gene involved in DNA synthesis. TSD2 was found to be cell cycle-regulated and mRNA levels peaked in early S or G1 phase.

Alternate JournalMol. Gen. Genet.
PubMed ID9037106
Grant ListGM42482 / GM / NIGMS NIH HHS / United States

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