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Metabolomics of Mycobacterium tuberculosis reveals compartmentalized co-catabolism of carbon substrates.

TitleMetabolomics of Mycobacterium tuberculosis reveals compartmentalized co-catabolism of carbon substrates.
Publication TypeJournal Article
Year of Publication2010
Authorsde Carvalho LPedro S, Fischer SM, Marrero J, Nathan C, Ehrt S, Rhee KY
JournalChem Biol
Date Published2010 Oct 29
KeywordsCarbon, Catabolite Repression, Glycolysis, Metabolome, Metabolomics, Mycobacterium tuberculosis

Metabolic adaptation to the host environment is a defining feature of the pathogenicity of Mycobacterium tuberculosis (Mtb), but we lack biochemical knowledge of its metabolic networks. Many bacteria use catabolite repression as a regulatory mechanism to maximize growth by consuming individual carbon substrates in a preferred sequence and growing with diauxic kinetics. Surprisingly, untargeted metabolite profiling of Mtb growing on ¹³C-labeled carbon substrates revealed that Mtb could catabolize multiple carbon sources simultaneously to achieve enhanced monophasic growth. Moreover, when co-catabolizing multiple carbon sources, Mtb differentially catabolized each carbon source through the glycolytic, pentose phosphate, and/or tricarboxylic acid pathways to distinct metabolic fates. This unusual topologic organization of bacterial intermediary metabolism has not been previously observed and may subserve the pathogenicity of Mtb.

Alternate JournalChem Biol
PubMed ID21035735

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