|Metabolomics of Mycobacterium tuberculosis reveals compartmentalized co-catabolism of carbon substrates.
|Year of Publication
|de Carvalho LPedro S, Fischer SM, Marrero J, Nathan C, Ehrt S, Rhee KY
|2010 Oct 29
|Carbon, Catabolite Repression, Glycolysis, Metabolome, Metabolomics, Mycobacterium tuberculosis
Metabolic adaptation to the host environment is a defining feature of the pathogenicity of Mycobacterium tuberculosis (Mtb), but we lack biochemical knowledge of its metabolic networks. Many bacteria use catabolite repression as a regulatory mechanism to maximize growth by consuming individual carbon substrates in a preferred sequence and growing with diauxic kinetics. Surprisingly, untargeted metabolite profiling of Mtb growing on ¹³C-labeled carbon substrates revealed that Mtb could catabolize multiple carbon sources simultaneously to achieve enhanced monophasic growth. Moreover, when co-catabolizing multiple carbon sources, Mtb differentially catabolized each carbon source through the glycolytic, pentose phosphate, and/or tricarboxylic acid pathways to distinct metabolic fates. This unusual topologic organization of bacterial intermediary metabolism has not been previously observed and may subserve the pathogenicity of Mtb.
Submitted by jom4013 on December 3, 2020 - 3:28pm