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Isolation of the REC1 gene controlling recombination in Ustilago maydis.

TitleIsolation of the REC1 gene controlling recombination in Ustilago maydis.
Publication TypeJournal Article
Year of Publication1989
AuthorsTsukuda T, Bauchwitz R, Holloman WK
JournalGene
Volume85
Issue2
Pagination335-41
Date Published1989 Dec 28
ISSN0378-1119
KeywordsAlleles, Basidiomycota, Cloning, Molecular, DNA Repair, DNA, Fungal, Genes, Fungal, Genotype, Kinetics, Mutation, Recombination, Genetic, Restriction Mapping, Ultraviolet Rays, Ustilago
Abstract

Previous studies on the rec1 mutant of Ustilago maydis revealed a complex phenotype. The mutant was found to be defective in DNA repair, recombination, growth, mutagenesis, and meiosis. We cloned the REC1 gene by complementing the phenotype of the mutant using a genomic DNA library made in a replicating vector and provided proof of identity by one-step gene disruption of the wild-type (wt) REC1 locus with a mutant allele constructed in vitro. The transcript of the REC1 gene was identified as a damage-inducible 1.7-kb RNA. After UV irradiation, the level of the transcript was observed to rise ten- to 15-fold. In the rec1 mutant, the basal level of the 1.7-kb transcript was tenfold lower than in wt. In addition, a second transcript of 1.1 kb was noted in rec1-1 cells in response to DNA damage.

Alternate JournalGene
PubMed ID2628171
Grant ListGM42482 / GM / NIGMS NIH HHS / United States

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