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Interleukin-10 expression in macrophages during phagocytosis of apoptotic cells is mediated by homeodomain proteins Pbx1 and Prep-1.

TitleInterleukin-10 expression in macrophages during phagocytosis of apoptotic cells is mediated by homeodomain proteins Pbx1 and Prep-1.
Publication TypeJournal Article
Year of Publication2007
AuthorsChung EY, Liu J, Homma Y, Zhang Y, Brendolan A, Saggese M, Han J, Silverstein R, Selleri L, Ma X
JournalImmunity
Volume27
Issue6
Pagination952-64
Date Published2007 Dec
ISSN1074-7613
KeywordsAnimals, Apoptosis, CD36 Antigens, DNA, DNA-Binding Proteins, Homeodomain Proteins, Humans, Interleukin-10, Macrophages, Mice, Mice, Inbred C57BL, p38 Mitogen-Activated Protein Kinases, Phagocytosis, Pre-B-Cell Leukemia Transcription Factor 1, Promoter Regions, Genetic, Proto-Oncogene Proteins, Transcription Factors, Transcription, Genetic
Abstract

Production of interleukin (IL)-10, a major immunoregulatory cytokine, by phagocytes during clearance of apoptotic cells is critical to ensuring cellular homeostasis and suppression of autoimmunity. Little is known about the regulatory mechanisms in this fundamental process. We report that IL-10 production stimulated by apoptotic cells was regulated at the point of transcription in a manner dependent on p38 mitogen-activated protein kinase, partially on the scavenger receptor CD36, and required cell-cell contact but not phagocytosis. By using a reporter assay, we mapped the apoptotic-cell-response element (ACRE) in the human IL10 promoter and provide biochemical and physiological evidence that ACRE mediates the transcriptional activation of IL10 by pre-B cell leukemia transcription factor-1b and another Hox cofactor Pbx-regulating protein 1 in response to apoptotic cells. This study establishes a role of two developmentally critical factors (Pbx1 and Prep-1) in the regulation of homeostasis in the immune system.

DOI10.1016/j.immuni.2007.11.014
Alternate JournalImmunity
PubMed ID18093541
PubMed Central IDPMC2194654
Grant ListAI45899 / AI / NIAID NIH HHS / United States
R01 AI045899-07 / AI / NIAID NIH HHS / United States
R01 AI045899-09 / AI / NIAID NIH HHS / United States
R01 AI045899 / AI / NIAID NIH HHS / United States
R01 AI045899-10 / AI / NIAID NIH HHS / United States
R01 AI045899-08 / AI / NIAID NIH HHS / United States

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