Interchangeable RNA polymerase I and II enhancers.

TitleInterchangeable RNA polymerase I and II enhancers.
Publication TypeJournal Article
Year of Publication1990
AuthorsLorch Y, Lue NF, Kornberg RD
JournalProc Natl Acad Sci U S A
Date Published1990 Nov
KeywordsBase Sequence, Enhancer Elements, Genetic, Escherichia coli, Genetic Vectors, Molecular Sequence Data, Oligonucleotide Probes, Plasmids, Restriction Mapping, RNA Polymerase I, RNA Polymerase II, Saccharomyces cerevisiae

The RNA polymerase I (pol I) enhancer of Saccharomyces cerevisiae contains at least three elements commonly associated with RNA polymerase II (pol II) enhancers, binding sites for the transcriptional activators general regulatory factor 2 and autonomously replicating sequence-binding factor I, and a thymidine-rich element. When the particular form of the thymidine-rich element found in the pol I enhancer was placed in front of a pol II promoter, transcription was stimulated 43-fold, comparable to the effect of a powerful pol II activator such as Gal4. Conversely, when two copies of a thymidine-rich element from a pol II enhancer were placed upstream of a pol I promoter, transcription was stimulated 38-fold. This functional reciprocity of pol I and II enhancers may reflect similarities in the mechanisms of transcriptional activation. The pol I enhancer also contains an element that appears to be pol I-specific and prevent the activation of pol II.

Alternate JournalProc Natl Acad Sci U S A
PubMed ID2236033
PubMed Central IDPMC54923
Grant ListGM36659 / GM / NIGMS NIH HHS / United States

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