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Interchangeable RNA polymerase I and II enhancers.

TitleInterchangeable RNA polymerase I and II enhancers.
Publication TypeJournal Article
Year of Publication1990
AuthorsLorch Y, Lue NF, Kornberg RD
JournalProc Natl Acad Sci U S A
Volume87
Issue21
Pagination8202-6
Date Published1990 Nov
ISSN0027-8424
KeywordsBase Sequence, Enhancer Elements, Genetic, Escherichia coli, Genetic Vectors, Molecular Sequence Data, Oligonucleotide Probes, Plasmids, Restriction Mapping, RNA Polymerase I, RNA Polymerase II, Saccharomyces cerevisiae
Abstract

The RNA polymerase I (pol I) enhancer of Saccharomyces cerevisiae contains at least three elements commonly associated with RNA polymerase II (pol II) enhancers, binding sites for the transcriptional activators general regulatory factor 2 and autonomously replicating sequence-binding factor I, and a thymidine-rich element. When the particular form of the thymidine-rich element found in the pol I enhancer was placed in front of a pol II promoter, transcription was stimulated 43-fold, comparable to the effect of a powerful pol II activator such as Gal4. Conversely, when two copies of a thymidine-rich element from a pol II enhancer were placed upstream of a pol I promoter, transcription was stimulated 38-fold. This functional reciprocity of pol I and II enhancers may reflect similarities in the mechanisms of transcriptional activation. The pol I enhancer also contains an element that appears to be pol I-specific and prevent the activation of pol II.

DOI10.1073/pnas.87.21.8202
Alternate JournalProc Natl Acad Sci U S A
PubMed ID2236033
PubMed Central IDPMC54923
Grant ListGM36659 / GM / NIGMS NIH HHS / United States

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