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Interaction of GAL4 and GAL80 gene regulatory proteins in vitro.

TitleInteraction of GAL4 and GAL80 gene regulatory proteins in vitro.
Publication TypeJournal Article
Year of Publication1987
AuthorsLue NF, Chasman DI, Buchman AR, Kornberg RD
JournalMol Cell Biol
Volume7
Issue10
Pagination3446-51
Date Published1987 Oct
ISSN0270-7306
KeywordsDNA, Fungal, DNA-Binding Proteins, Fungal Proteins, Galactose, Gene Expression Regulation, Immunologic Techniques, Macromolecular Substances, Protein Binding, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Transcription Factors
Abstract

The GAL80 protein of Saccharomyces cerevisiae, synthesized in vitro, bound tightly to GAL4 protein and to a GAL4 protein-upstream activation sequence DNA complex, as shown by (i) coimmunoprecipitation of GAL4 and GAL80 proteins with anti-GAL4 antiserum, (ii) an electrophoretic mobility shift of a GAL4 protein-upstream activation sequence DNA complex upon the addition of GAL80 protein, and (iii) GAL4-dependent binding of GAL80 protein to upstream activation sequence DNA immobilized on Sepharose beads. Anti-GAL4 antisera were raised against a GAL4-URA3 fusion protein, which could be purified to homogeneity in a single step with the use of an affinity chromatographic procedure for the URA3 gene product.

DOI10.1128/mcb.7.10.3446
Alternate JournalMol Cell Biol
PubMed ID3316976
PubMed Central IDPMC367995
Grant ListGM-36659 / GM / NIGMS NIH HHS / United States

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