Submitted by jom4013 on December 3, 2020 - 4:18pm
| Title | Interaction of GAL4 and GAL80 gene regulatory proteins in vitro. |
| Publication Type | Journal Article |
| Year of Publication | 1987 |
| Authors | Lue NF, Chasman DI, Buchman AR, Kornberg RD |
| Journal | Mol Cell Biol |
| Volume | 7 |
| Issue | 10 |
| Pagination | 3446-51 |
| Date Published | 1987 Oct |
| ISSN | 0270-7306 |
| Keywords | DNA, Fungal, DNA-Binding Proteins, Fungal Proteins, Galactose, Gene Expression Regulation, Immunologic Techniques, Macromolecular Substances, Protein Binding, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Transcription Factors |
| Abstract | The GAL80 protein of Saccharomyces cerevisiae, synthesized in vitro, bound tightly to GAL4 protein and to a GAL4 protein-upstream activation sequence DNA complex, as shown by (i) coimmunoprecipitation of GAL4 and GAL80 proteins with anti-GAL4 antiserum, (ii) an electrophoretic mobility shift of a GAL4 protein-upstream activation sequence DNA complex upon the addition of GAL80 protein, and (iii) GAL4-dependent binding of GAL80 protein to upstream activation sequence DNA immobilized on Sepharose beads. Anti-GAL4 antisera were raised against a GAL4-URA3 fusion protein, which could be purified to homogeneity in a single step with the use of an affinity chromatographic procedure for the URA3 gene product. |
| DOI | 10.1128/mcb.7.10.3446 |
| Alternate Journal | Mol Cell Biol |
| PubMed ID | 3316976 |
| PubMed Central ID | PMC367995 |
| Grant List | GM-36659 / GM / NIGMS NIH HHS / United States |