|Interaction between Ustilago maydis REC2 and RAD51 genes in DNA repair and mitotic recombination.
|Year of Publication
|Ferguson DO, Rice MC, Rendi MH, Kotani H, Kmiec EB, Holloman WK
|Amino Acid Sequence, Base Sequence, Chromosomes, Fungal, DNA Repair, DNA, Fungal, DNA-Binding Proteins, Fungal Proteins, Gamma Rays, Gene Expression Regulation, Genes, Fungal, Mitosis, Molecular Sequence Data, Plasmids, Rad51 Recombinase, Recombination, Genetic, Ultraviolet Rays, Ustilago
A gene encoding a Ustilago maydis Rad51 orthologue has been isolated, rad51-1, a mutant constructed by disrupting the gene, was as sensitive to killing by ultraviolet light and gamma radiation as the rec2-1 mutant and slightly more sensitive to killing by methyl methanesulfonate. There was no suppression of killing by ultraviolet light when a rec2-1 strain was transformed with a multicopy plasmid containing RAD51, nor was there suppression when rad51-1 was transformed with a multicopy plasmid containing REC2. Recombination proficiency as measured by a gap repair assay was diminished in both rec2-1 and rad51-1 strains. In rec2-1 the frequency of recombination was decreased, but the spectrum of events was similar to that observed in wild type, while in rad51-1 the frequency as well as the spectrum of recombination events were different. Studies with the rec2-1 rad51-1 double mutant indicated that there was epistasis in the action of REC2 and RAD51 in certain repair and recombination functions, but some measure of independent action in other functions.
|PubMed Central ID
|GM-53732 / GM / NIGMS NIH HHS / United States
Submitted by alp2017 on April 24, 2015 - 10:41am