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Initiation by yeast RNA polymerase II at the adenoviral major late promoter in vitro.

TitleInitiation by yeast RNA polymerase II at the adenoviral major late promoter in vitro.
Publication TypeJournal Article
Year of Publication1989
AuthorsLue NF, Flanagan PM, Sugimoto K, Kornberg RD
JournalScience
Volume246
Issue4930
Pagination661-4
Date Published1989 Nov 03
ISSN0036-8075
KeywordsAdenoviruses, Human, Base Sequence, Genes, Fungal, GTP-Binding Proteins, HeLa Cells, Humans, Molecular Sequence Data, Oligonucleotide Probes, Promoter Regions, Genetic, RNA Polymerase II, Saccharomyces cerevisiae, Templates, Genetic, Transcription, Genetic
Abstract

Transcription of the yeast CYC1 promoter fused to a sequence lacking guanosine residues provided a rapid, sensitive assay of initiation by RNA polymerase II in yeast extracts. Initiation was enhanced by yeast and mammalian activator proteins. The adenoviral major late promoter fused to the G-minus sequence was transcribed in yeast extracts with an efficiency comparable to that observed in HeLa extracts, showing that promoters as well as transcription factors are functionally interchangeable across species. Initiation occurred at different sites, approximately 30 and 63 to 69 base pairs downstream of the TATA element of the adenoviral promoter in HeLa and yeast extracts, respectively, distances characteristic of initiation in the two systems in vivo. A component of the transcription system and not the promoter sequence determines the distance to the initiation site.

DOI10.1126/science.2510298
Alternate JournalScience
PubMed ID2510298
Grant ListGM36659 / GM / NIGMS NIH HHS / United States

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