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Microbiology and Immunology

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Heteroduplex formation and polarity during strand transfer promoted by Ustilago rec 1 protein.

TitleHeteroduplex formation and polarity during strand transfer promoted by Ustilago rec 1 protein.
Publication TypeJournal Article
Year of Publication1983
AuthorsKmiec EB, Holloman WK
JournalCell
Volume33
Issue3
Pagination857-64
Date Published1983 Jul
ISSN0092-8674
KeywordsAdenosine Triphosphate, Bacterial Proteins, Basidiomycota, DNA, Fungal, Exodeoxyribonuclease V, Fungal Proteins, Rec A Recombinases, Recombination, Genetic, Ustilago
Abstract

Ustilago rec1 protein promotes formation of joint molecules between circular strands and linear duplex DNA in two stages. During the first stage--synapsis--ATP, or the nonhydrolyzable analog adenylyl-imidodiphosphate, is a necessary cofactor in the formation of complexes. During the second stage--strand exchange--transfer of the circular single strand to the duplex appears to require energy derived from ATP hydrolysis. Rec1-promoted strand exchange is driven in a unique direction. Circular plus strand pairs with the 5' end of its complement in the linear duplex molecule. The 3' end of the plus strand is displaced from the duplex as the circle rolls in.

Alternate JournalCell
PubMed ID6871997

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