| Title | Generation of conditional mutants in higher eukaryotes by switching between the expression of two genes. |
| Publication Type | Journal Article |
| Year of Publication | 1999 |
| Authors | Baron U, Schnappinger D, Helbl V, Gossen M, Hillen W, Bujard H |
| Journal | Proc Natl Acad Sci U S A |
| Volume | 96 |
| Issue | 3 |
| Pagination | 1013-8 |
| Date Published | 1999 Feb 02 |
| ISSN | 0027-8424 |
| Keywords | Bacterial Proteins, DNA-Binding Proteins, Doxorubicin, Gene Expression Regulation, Bacterial, Genes, Switch, HeLa Cells, Humans, Phenotype, Promoter Regions, Genetic, Recombinant Fusion Proteins, Tetracycline, Trans-Activators, Transcriptional Activation, Transfection |
| Abstract | A regulatory system for the in-depth study of gene functions in higher eukaryotic cells has been developed. It is based on the tetracycline-controlled transactivators and reverse tTA, which were remodeled to discriminate efficiently between two different promoters. The system permits one to control reversibly the activity of two genes, or two alleles of a gene, in a mutually exclusive way, and also allows one to abrogate the activities of both. This dual regulatory circuit, which can be operated by a single effector substance such as doxycycline, overcomes limitations of conventional genetic approaches. The conditional mutants that can now be generated will be useful for the study of gene function in vitro and in vivo. In addition, the system may be of value for a variety of practical applications, including gene therapy. |
| DOI | 10.1073/pnas.96.3.1013 |
| Alternate Journal | Proc Natl Acad Sci U S A |
| PubMed ID | 9927685 |
| PubMed Central ID | PMC15342 |
Submitted by jom4013 on December 3, 2020 - 4:05pm