For COVID-19 vaccine updates, please review our information guide. For patient eligibility and scheduling availability, please visit VaccineTogetherNY.org.

A GAL family of upstream activating sequences in yeast: roles in both induction and repression of transcription.

TitleA GAL family of upstream activating sequences in yeast: roles in both induction and repression of transcription.
Publication TypeJournal Article
Year of Publication1986
AuthorsBram RJ, Lue NF, Kornberg RD
JournalEMBO J
Volume5
Issue3
Pagination603-8
Date Published1986 Mar
ISSN0261-4189
KeywordsBase Sequence, DNA Restriction Enzymes, Fungal Proteins, Galactose, Genes, Genes, Fungal, Genes, Regulator, Plasmids, Saccharomyces cerevisiae, Transcription, Genetic
Abstract

Binding sites for the GAL4-positive regulatory protein have been identified upstream of six galactose-inducible genes of Saccharomyces cerevisiae on the basis of (i) protection in DNAse I footprints, (ii) loss of protection when excess GAL4-binding oligonucleotide is added and (iii) homology with a 23-bp dyad-symmetric consensus sequence. Many of the binding sites have been shown to function as upstream activating sequences. The number of binding sites upstream of the various genes ranges from one to four, but a feature is conserved: in cases of multiple sites there is a pair with highest binding affinity located at dyad--dyad distances of 82--87 bp. We suggest that a pair of sites facilitates repression by the GAL80-negative regulatory protein, on the basis of (i) a correlation of a pair of sites (or only one) with full (or only partial) repression and (ii) the introduction of a second site abolishing transcription occurring with one.

Alternate JournalEMBO J
PubMed ID3011415
PubMed Central IDPMC1166805
Grant ListGM07365 / GM / NIGMS NIH HHS / United States

Weill Cornell Medicine Microbiology and Immunology 1300 York Avenue, Box 62 New York, NY 10065 Phone: (212) 746-6505 Fax: (212) 746-8587