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Expression of thyrotropin-releasing hormone receptors by adenovirus-mediated gene transfer reveals that thyrotropin-releasing hormone desensitization is cell specific.

TitleExpression of thyrotropin-releasing hormone receptors by adenovirus-mediated gene transfer reveals that thyrotropin-releasing hormone desensitization is cell specific.
Publication TypeJournal Article
Year of Publication1994
AuthorsFalck-Pedersen E, Heinflink M, Alvira M, Nussenzveig DR, Gershengorn MC
JournalMol Pharmacol
Volume45
Issue4
Pagination684-9
Date Published1994 Apr
ISSN0026-895X
KeywordsAdenoviridae, Animals, Down-Regulation, Gene Transfer Techniques, Genetic Vectors, In Vitro Techniques, Mice, Receptors, Thyrotropin-Releasing Hormone, Tetradecanoylphorbol Acetate, Thyrotropin-Releasing Hormone
Abstract

Biological studies of seven-transmembrane region G protein-coupled receptors have been restricted by available techniques for gene transfer into mammalian cells. We have created a highly efficient adenovirus-based expression vector for the thyrotropin-releasing hormone (TRH) receptor (TRH-R), AdCMVmTRHR, to circumvent difficulties encountered when transient or stable plasmid expression systems are used. We show that infection with AdCMVmTRHR results in fully functional TRH-Rs, which can be expressed in a broad range of mammalian cell types, including those resistant to conventional transient transfection. TRH-Rs can be expressed at high levels, up to 2 x 10(6) receptors/cell. Expression in several cell lines in culture reveals that rapid TRH-R desensitization by TRH and phorbol 12-myristate 13-acetate is cell type specific. The versatility of adenovirus-mediated gene transfer and expression of TRH-Rs not only facilitates in vitro studies of TRH-R biology but also provides a valuable in vivo expression vector capable of extending TRH-R studies to animal model systems.

Alternate JournalMol Pharmacol
PubMed ID8183247
Grant ListDK43046 / DK / NIDDK NIH HHS / United States
GM41967 / GM / NIGMS NIH HHS / United States

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