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Construction of conditional knockdown mutants in mycobacteria.

TitleConstruction of conditional knockdown mutants in mycobacteria.
Publication TypeJournal Article
Year of Publication2015
AuthorsSchnappinger D, O'Brien KM, Ehrt S
JournalMethods Mol Biol
Volume1285
Pagination151-75
Date Published2015
ISSN1940-6029
KeywordsGene Knockdown Techniques, Gene Targeting, Genetic Engineering, Mutation, Mycobacterium, Plasmids, Promoter Regions, Genetic
Abstract

By definition, essential genes are fundamental to bacterial growth, yet the functions of many such genes remain unknown. Essential genes furthermore are central to the activity of most antibacterial drugs and among the most attractive targets for the development of new therapeutics. This chapter describes how synthetic genetic switches that utilize transcriptional repression, controlled proteolysis, or both to silence gene activity can be applied to construct and characterize conditional knockdown (cKD) mutants for essential genes in Mycobacterium smegmatis and Mycobacterium tuberculosis.

DOI10.1007/978-1-4939-2450-9_9
Alternate JournalMethods Mol Biol
PubMed ID25779315

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