Construction and characterization of hexon-chimeric adenoviruses: specification of adenovirus serotype.

TitleConstruction and characterization of hexon-chimeric adenoviruses: specification of adenovirus serotype.
Publication TypeJournal Article
Year of Publication1998
AuthorsGall JG, Crystal RG, Falck-Pedersen E
JournalJ Virol
Date Published1998 Dec
KeywordsAdenoviridae Infections, Animals, Antibodies, Viral, Antigens, Viral, Capsid, Capsid Proteins, Cell Line, Chimera, Cross Reactions, Epitopes, Genes, Viral, Genetic Vectors, Humans, Mastadenovirus, Neutralization Tests, Rats, Rats, Sprague-Dawley, Recombination, Genetic, Serotyping, Transduction, Genetic

This study has used the strategy of gene replacement to characterize the contribution of the adenovirus (Ad) capsid protein hexon to serotype definition. By replacing the Ad type 5 (Ad5) hexon gene with sequences from Ad2, we have changed the type specificity of the chimeric virus. The type-determining epitopes are primarily associated with loop 1 of hexon and, to a much lesser degree, with loop 2. In spite of the serotype distinctiveness of the chimeric hexon viruses, epitope similarity between the vectors resulted in a low level of cross-reactive neutralizing antibody, which in combination with activated cellular and innate arms of the immune system is sufficient to suppress gene transduction following readministration in vivo.

Alternate JournalJ Virol
PubMed ID9811772
PubMed Central IDPMC110610
Grant ListP01 HL051746 / HL / NHLBI NIH HHS / United States
P01 HL51746 / HL / NHLBI NIH HHS / United States

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