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Construction and characterization of hexon-chimeric adenoviruses: specification of adenovirus serotype.

TitleConstruction and characterization of hexon-chimeric adenoviruses: specification of adenovirus serotype.
Publication TypeJournal Article
Year of Publication1998
AuthorsGall JG, Crystal RG, Falck-Pedersen E
JournalJ Virol
Volume72
Issue12
Pagination10260-4
Date Published1998 Dec
ISSN0022-538X
KeywordsAdenoviridae Infections, Animals, Antibodies, Viral, Antigens, Viral, Capsid, Capsid Proteins, Cell Line, Chimera, Cross Reactions, Epitopes, Genes, Viral, Genetic Vectors, Humans, Mastadenovirus, Neutralization Tests, Rats, Rats, Sprague-Dawley, Recombination, Genetic, Serotyping, Transduction, Genetic
Abstract

This study has used the strategy of gene replacement to characterize the contribution of the adenovirus (Ad) capsid protein hexon to serotype definition. By replacing the Ad type 5 (Ad5) hexon gene with sequences from Ad2, we have changed the type specificity of the chimeric virus. The type-determining epitopes are primarily associated with loop 1 of hexon and, to a much lesser degree, with loop 2. In spite of the serotype distinctiveness of the chimeric hexon viruses, epitope similarity between the vectors resulted in a low level of cross-reactive neutralizing antibody, which in combination with activated cellular and innate arms of the immune system is sufficient to suppress gene transduction following readministration in vivo.

DOI10.1128/JVI.72.12.10260-10264.1998
Alternate JournalJ Virol
PubMed ID9811772
PubMed Central IDPMC110610
Grant ListP01 HL051746 / HL / NHLBI NIH HHS / United States
P01 HL51746 / HL / NHLBI NIH HHS / United States

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