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Construction of an adenovirus type 7a E1A- vector.

TitleConstruction of an adenovirus type 7a E1A- vector.
Publication TypeJournal Article
Year of Publication1997
AuthorsAbrahamsen K, Kong HL, Mastrangeli A, Brough D, Lizonova A, Crystal RG, Falck-Pedersen E
JournalJ Virol
Volume71
Issue11
Pagination8946-51
Date Published1997 Nov
ISSN0022-538X
KeywordsAdenovirus E1A Proteins, Adenoviruses, Human, Animals, Cells, Cultured, Defective Viruses, Gene Expression Regulation, Viral, Gene Transfer Techniques, Genetic Vectors, Humans, Mice, Transduction, Genetic, Virus Replication
Abstract

A strategy for constructing replication-defective adenovirus vectors from non-subgroup C viruses has been successfully demonstrated with adenovirus type 7 strain a (Ad7a) as the prototype. An E1A-deleted Ad7a reporter virus expressing the chloramphenicol acetyltransferase (CAT) gene from the cytomegalovirus promoter enhancer was constructed with DNA fragments isolated from Ad7a, an Ad7a recombination reporter plasmid, and the 293 cell line. The Ad7a-CAT virus particle transduces A549 cells as efficiently as Ad5-based vectors. Intravenous infections in a murine model indicate that the Ad7a-CAT virus infects a variety of tissues, with maximal levels of CAT gene expression found in the liver. The duration of Ad7a-CAT transgene expression in the liver was maximally maintained 2 weeks postinfection, with a decline to baseline activity by the week 4 postinfection. Ad7a-CAT represents the first example of a non-subgroup C E1A- adenovirus gene transfer vector.

DOI10.1128/JVI.71.11.8946-8951.1997
Alternate JournalJ Virol
PubMed ID9343264
PubMed Central IDPMC192370
Grant ListP01 HL51746 / HL / NHLBI NIH HHS / United States

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