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Cloning and disruption of Ustilago maydis genes.

TitleCloning and disruption of Ustilago maydis genes.
Publication TypeJournal Article
Year of Publication1989
AuthorsFotheringham S, Holloman WK
JournalMol Cell Biol
Volume9
Issue9
Pagination4052-5
Date Published1989 Sep
ISSN0270-7306
KeywordsBasidiomycota, Cloning, Molecular, Genes, Fungal, Genetic Complementation Test, Genetic Markers, Genetic Vectors, Mutation, Plasmids, Transformation, Genetic, Ustilago
Abstract

We have demonstrated that genes from Ustilago maydis can be cloned by direct complementation of mutants through the use of genomic libraries made in a high-frequency transformation vector. We isolated a gene involved in amino acid biosynthesis as an illustrative example and showed that integrative and one-step disruption methods can be used to create null mutations in the chromosomal copy of the gene by homologous recombination. The results of this investigation make it clear that one-step gene disruption will be of general utility in investigations of U. maydis, since simple, precise replacement of the sequence under study was readily achieved.

Alternate JournalMol. Cell. Biol.
PubMed ID2779577
PubMed Central IDPMC362470
Grant ListGM 36327 / GM / NIGMS NIH HHS / United States

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