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Cloning and characterization of inducible nitric oxide synthase from mouse macrophages.

TitleCloning and characterization of inducible nitric oxide synthase from mouse macrophages.
Publication TypeJournal Article
Year of Publication1992
AuthorsXie QW, Cho HJ, Calaycay J, Mumford RA, Swiderek KM, Lee TD, Ding A, Troso T, Nathan C
JournalScience
Volume256
Issue5054
Pagination225-8
Date Published1992 Apr 10
ISSN0036-8075
KeywordsAmino Acid Oxidoreductases, Amino Acid Sequence, Animals, Base Sequence, Cell Line, Cells, Cultured, Cloning, Molecular, Codon, Enzyme Induction, Interferon-gamma, Isoenzymes, Kinetics, Lipopolysaccharides, Macrophages, Mammary Neoplasms, Experimental, Mice, Molecular Sequence Data, Molecular Weight, Neutrophils, Nitric Oxide Synthase, Oligodeoxyribonucleotides, Poly A, Rats, RNA, RNA, Messenger, Sequence Homology, Nucleic Acid, Transcription, Genetic
Abstract

Nitric oxide (NO) conveys a variety of messages between cells, including signals for vasorelaxation, neurotransmission, and cytotoxicity. In some endothelial cells and neurons, a constitutive NO synthase is activated transiently by agonists that elevate intracellular calcium concentrations and promote the binding of calmodulin. In contrast, in macrophages, NO synthase activity appears slowly after exposure of the cells to cytokines and bacterial products, is sustained, and functions independently of calcium and calmodulin. A monospecific antibody was used to clone complementary DNA that encoded two isoforms of NO synthase from immunologically activated mouse macrophages. Liquid chromatography-mass spectrometry was used to confirm most of the amino acid sequence. Macrophage NO synthase differs extensively from cerebellar NO synthase. The macrophage enzyme is immunologically induced at the transcriptional level and closely resembles the enzyme in cytokine-treated tumor cells and inflammatory neutrophils.

Alternate JournalScience
PubMed ID1373522
Grant ListAI30165 / AI / NIAID NIH HHS / United States
CA43610 / CA / NCI NIH HHS / United States

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