Cloning and analysis of the locus for mosquito vitellogenic carboxypeptidase.

TitleCloning and analysis of the locus for mosquito vitellogenic carboxypeptidase.
Publication TypeJournal Article
Year of Publication1993
AuthorsDeitsch K, Raikhel AS
JournalInsect Mol Biol
Date Published1993
KeywordsAedes, Animals, Base Sequence, Binding Sites, Carboxypeptidases, Chromosome Mapping, Cloning, Molecular, DNA, Fat Body, Female, Insect Proteins, Molecular Sequence Data, Peptide Chain Initiation, Translational, Protein Binding, Rats

Vitellogenic carboxypeptidase is a 53 kDa yolk protein produced by the fat body of the female mosquito, Aedes aegypti, in response to a blood meal. Its expression is sex-, stage- and tissue-specific and is identical to that of the major yolk protein, vitellogenin. The gene is intronless and two alleles have been cloned and sequenced, including more than 1.5 kb on both sides of the coding region. A capsite consensus recently identified as an arthropod initiator is present at the start site of transcription. Upstream of this capsite is a 16 bp imperfect palindrome repeated four times showing strong homology to defined hormone-response elements. In addition, a region that closely resembles the fat body enhancer and double sex binding site from the Drosophila yolk protein genes and several potential fat body-specific regulatory protein binding sites were found.

Alternate JournalInsect Mol. Biol.
PubMed ID9087558
Grant ListAI-24716 / AI / NIAID NIH HHS / United States

Weill Cornell Medicine Microbiology and Immunology 1300 York Avenue, Box 62 New York, NY 10065 Phone: (212) 746-6505 Fax: (212) 746-8587