Characterization of a potent dominant negative mutant variant of Rad51 in Ustilago maydis.

TitleCharacterization of a potent dominant negative mutant variant of Rad51 in Ustilago maydis.
Publication TypeJournal Article
Year of Publication2019
AuthorsSutherland JH, Holloman WK
JournalDNA Repair (Amst)
Date Published2019 06
KeywordsAlleles, Amino Acid Sequence, DNA Damage, Mutant Proteins, Mutation, Phenotype, Rad51 Recombinase, Ustilago

Rad51 serves to maintain and protect integrity of the genome through its actions in DNA repair and replication fork protection. The active form of Rad51 is a nucleoprotein filament consisting of chains of protomer units arranged linearly along single-stranded DNA. In a mutant screen using Ustilago maydis as an experimental system we identified a novel variant of Rad51, in which an amino acid change near the protomer-protomer interaction interface confers a strong trans dominant inhibitory effect on resistance to DNA damaging agents and proficiency in homologous recombination. Modeling studies of the mutated residue D161Y suggested that steric interference with surrounding residues was the likely cause of the inhibitory effect. Changes of two nearby residues, predicted from the modeling to minimize steric clashes, mitigated the inhibition of DNA repair. Direct testing of purified Rad51 protein in defined biochemical reactions revealed it to be devoid of DNA-binding activity itself, but capable of interfering with Rad51 in formation and maintenance of nucleoprotein filaments on single-stranded DNA and in DNA strand exchange. Rad51 protein appears to be unable to self-associate in solution and defective in forming complexes with the U. maydis BRCA2 ortholog.

Alternate JournalDNA Repair (Amst)
PubMed ID31005682

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