For COVID-19 vaccine updates, please review our information guide. For patient eligibility and scheduling availability, please visit VaccineTogetherNY.org.

Brh2 and Rad51 promote telomere maintenance in Ustilago maydis, a new model system of DNA repair proteins at telomeres.

TitleBrh2 and Rad51 promote telomere maintenance in Ustilago maydis, a new model system of DNA repair proteins at telomeres.
Publication TypeJournal Article
Year of Publication2013
AuthorsYu EYoung, Kojic M, Holloman WK, Lue NF
JournalDNA Repair (Amst)
Volume12
Issue7
Pagination472-9
Date Published2013 Jul
ISSN1568-7856
KeywordsFungal Proteins, Mutation, Nuclear Proteins, Rad51 Recombinase, Recombinational DNA Repair, Telomere, Telomere Homeostasis, Ustilago
Abstract

Recent studies implicate a number of DNA repair proteins in mammalian telomere maintenance. However, because several key repair proteins in mammals are missing from the well-studied budding and fission yeast, their roles at telomeres cannot be modeled in standard fungi. In this report, we explored the dimorphic fungus Ustilago maydis as an alternative model for telomere research. This fungus, which belongs to the phylum Basidiomycota, has a telomere repeat unit that is identical to the mammalian repeat, as well as a constellation of DNA repair proteins that more closely mimic the mammalian collection. We showed that the two core components of homology-directed repair (HDR) in U. maydis, namely Brh2 and Rad51, both promote telomere maintenance in telomerase positive cells, just like in mammals. In addition, we found that Brh2 is localized to telomeres in vivo, suggesting that it acts directly at chromosome ends. We surveyed a series of mutants with DNA repair defects, and found many of them to have short telomeres. Our results indicate that factors involved in DNA repair are probably also needed for optimal telomere maintenance in U. maydis, and that this fungus is a useful alternative model system for telomere research.

DOI10.1016/j.dnarep.2013.04.027
Alternate JournalDNA Repair (Amst)
PubMed ID23726221
PubMed Central IDPMC3684436
Grant ListR01 GM042482 / GM / NIGMS NIH HHS / United States
R01 GM062631 / GM / NIGMS NIH HHS / United States
GM042482 / GM / NIGMS NIH HHS / United States

Weill Cornell Medicine Microbiology and Immunology 1300 York Avenue, Box 62 New York, NY 10065 Phone: (212) 746-6505 Fax: (212) 746-8587