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BRCA2 homolog required for proficiency in DNA repair, recombination, and genome stability in Ustilago maydis.

TitleBRCA2 homolog required for proficiency in DNA repair, recombination, and genome stability in Ustilago maydis.
Publication TypeJournal Article
Year of Publication2002
AuthorsKojic M, Kostrub CF, Buchman AR, Holloman WK
JournalMol Cell
Volume10
Issue3
Pagination683-91
Date Published2002 Sep
ISSN1097-2765
KeywordsAmino Acid Sequence, DNA Nucleotidyltransferases, DNA Repair, DNA-Binding Proteins, Fungal Proteins, Genes, BRCA2, Genes, Fungal, Humans, Karyotyping, Meiosis, Molecular Sequence Data, Rad51 Recombinase, Recombination, Genetic, Sequence Alignment, Two-Hybrid System Techniques, Ustilago
Abstract

In a screen for DNA repair-defective mutants in the fungus Ustilago maydis, a gene encoding a BRCA2 family member, designated here as Brh2, was identified. A brh2 null allele was found to be defective in allelic recombination, meiosis, and repair of gaps and ionizing radiation damage to the same extent as rad51. Frequent marker loss in meiosis and diploid formation suggested that genomic instability was associated with brh2. This notion was confirmed by molecular karyotype analysis, which revealed gross chromosomal alterations associated with brh2. Yeast two-hybrid analysis indicated interaction between Brh2 and Rad51. Recapitulation in U. maydis of defects in DNA repair and genome stability associated with brh2 means that the BRCA2 gene family is more widespread than previously thought.

Alternate JournalMol. Cell
PubMed ID12408834
Grant ListGM42482 / GM / NIGMS NIH HHS / United States

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