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ATP-dependent processivity of a telomerase activity from Saccharomyces cerevisiae.

TitleATP-dependent processivity of a telomerase activity from Saccharomyces cerevisiae.
Publication TypeJournal Article
Year of Publication1995
AuthorsLue NF, Wang JC
JournalJ Biol Chem
Volume270
Issue37
Pagination21453-6
Date Published1995 Sep 15
ISSN0021-9258
KeywordsAdenosine Triphosphate, Base Sequence, Cell Nucleus, Deoxyribonucleotides, DNA Nucleotidylexotransferase, DNA Primers, Kinetics, Molecular Sequence Data, Oligodeoxyribonucleotides, Ribonucleases, Saccharomyces cerevisiae, Substrate Specificity, Templates, Genetic
Abstract

Extracts of Saccharomyces cerevisiae were shown to support the elongation of oligodeoxynucleotides with telomere-like sequences. The primer sequence specificity of this elongation activity, its incorporation of dG and dT but not dA or dC from the corresponding triphosphates, and its sensitivity to RNase A and RNase H are all consistent with it being a telomerase. In contrast to the reported properties of other telomerases, the presence of ATP enhances the efficiency of initiation of the yeast enzyme and improves its processivity. Hydrolysis of ATP appears to be unnecessary for the observed effects, as the beta,gamma-imido or the gamma-thio derivative of ATP is nearly as effective.

DOI10.1074/jbc.270.37.21453
Alternate JournalJ Biol Chem
PubMed ID7665555
Grant ListGM24544 / GM / NIGMS NIH HHS / United States

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