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Analysis of mouse major urinary protein genes: variation between the exonic sequences of group 1 genes and a comparison with an active gene out with group 1 both suggest that gene conversion has occurred between MUP genes.

TitleAnalysis of mouse major urinary protein genes: variation between the exonic sequences of group 1 genes and a comparison with an active gene out with group 1 both suggest that gene conversion has occurred between MUP genes.
Publication TypeJournal Article
Year of Publication1985
AuthorsClark AJ, Chave-Cox A, Ma X, Bishop JO
JournalEMBO J
Volume4
Issue12
Pagination3167-71
Date Published1985 Dec 01
ISSN0261-4189
KeywordsAnimals, Base Sequence, Cell-Free System, Chromatography, Affinity, Cloning, Molecular, DNA, DNA Restriction Enzymes, Gene Conversion, Genes, Genetic Variation, Glycosides, Mice, Protein Biosynthesis, Protein Processing, Post-Translational, Proteins
Abstract

Here we compare the exonic sequences of four Group 1 mouse major urinary protein (MUP) genes and four Group 1 cDNA sequences. These define seven different nucleotide sequences which differ from each other by 0.35% of bases on average, and which would code for seven different MUP proteins that could probably be resolved physically into at least five classes. The sequences differ at 13 nucleotide positions and at six codons, and although they are closely related their descent cannot be described by a simple series of duplications. We also describe the sequence of another liver cDNA (pMUP15) which has diverged from the Group 1 consensus sequence in 14.6% of bases. The divergence is much greater over exons 1-3 than over exons 4-6, suggesting that an ancestral gene conversion event has occurred. pMUP15 also differs from the Group 1 genes in having a longer signal peptide sequence and a different splice configuration between exons 6 and 7. Unlike the Group 1 sequences, pMUP15 contains a potential N-linked glycosylation site. Other published work has shown that a shorter cDNA clone which is identical over their common sequence to pMUP15 codes for MUP proteins that are unusually large in size and acidic in pI. We show here that mouse urine does indeed contain a glycosylated MUP protein with those properties, presumably the product of the gene that corresponds to pMUP15.

Alternate JournalEMBO J
PubMed ID3004936
PubMed Central IDPMC554637

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