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Adeno-associated virus type 2 preferentially integrates single genome copies with defined breakpoints.

TitleAdeno-associated virus type 2 preferentially integrates single genome copies with defined breakpoints.
Publication TypeJournal Article
Year of Publication2014
AuthorsJanovitz T, Sadelain M, Falck-Pedersen E
JournalVirol J
Volume11
Pagination15
Date Published2014 Jan 27
ISSN1743-422X
KeywordsDependovirus, Genome, Human, Humans, Sequence Analysis, DNA, Virus Integration
Abstract

BACKGROUND: Adeno-associated virus (AAV) serotype 2 prevalently infects humans and is the only described eukaryotic virus that integrates site-preferentially. In a recent high throughput study, the genome wide distribution of AAV-2 integrants was determined using Integrant Capture Sequencing (IC-Seq). Additional insight regarding the integration of AAV-2 into human genomic DNA could be gleaned by low-throughput sequencing of complete viral-chromosomal junctions.

FINDINGS: In this study, 140 clones derived from Integrant-Capture Sequencing were sequenced. 100 met sequence inclusion criteria, and of these 39 contained validated junction sequences. These unique sequences were analyzed to investigate the structure and location of viral-chromosomal junctions.

CONCLUSIONS: Overall the low-throughput analysis confirmed the genome wide distribution profile gathered through the IC-Seq analysis. We found no unidentifiable sequence inserted at AAV-2 chromosomal junctions. Assessing both left and right ends of the AAV genome, viral breakpoints predominantly occurred in one hairpin of the inverted terminal repeat and AAV genomes were preferentially integrated as single copies.

DOI10.1186/1743-422X-11-15
Alternate JournalVirol J
PubMed ID24468291
PubMed Central IDPMC3918229
Grant ListR01 AI094050 / AI / NIAID NIH HHS / United States
T32 GM007739 / GM / NIGMS NIH HHS / United States
R01AI094050 / AI / NIAID NIH HHS / United States
T32GM07739 / GM / NIGMS NIH HHS / United States

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