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Activation of yeast RNA polymerase II transcription by a thymidine-rich upstream element in vitro.

TitleActivation of yeast RNA polymerase II transcription by a thymidine-rich upstream element in vitro.
Publication TypeJournal Article
Year of Publication1989
AuthorsLue NF, Buchman AR, Kornberg RD
JournalProc Natl Acad Sci U S A
Volume86
Issue2
Pagination486-90
Date Published1989 Jan
ISSN0027-8424
KeywordsBase Sequence, DNA, Fungal, Molecular Sequence Data, Plasmids, RNA Polymerase II, Saccharomyces cerevisiae, Templates, Genetic, Transcription, Genetic
Abstract

A thymidine-rich sequence upstream of the DED1 gene of Saccharomyces cerevisiae activated transcription of the CYC1 promoter by RNA polymerase II in vitro. Activation was inhibited by an excess of an oligonucleotide with the same but not a closely related thymidine-rich sequence, pointing to the involvement of a specific thymidine-rich element-binding factor. The extent of activation was as great as 30-fold and showed a similar distance and orientation dependence and a similar effect of deletions in vitro as in vivo.

DOI10.1073/pnas.86.2.486
Alternate JournalProc Natl Acad Sci U S A
PubMed ID2643115
PubMed Central IDPMC286495
Grant ListGM-36659 / GM / NIGMS NIH HHS / United States

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