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Accurate initiation at RNA polymerase II promoters in extracts from Saccharomyces cerevisiae.

TitleAccurate initiation at RNA polymerase II promoters in extracts from Saccharomyces cerevisiae.
Publication TypeJournal Article
Year of Publication1987
AuthorsLue NF, Kornberg RD
JournalProc Natl Acad Sci U S A
Volume84
Issue24
Pagination8839-43
Date Published1987 Dec
ISSN0027-8424
KeywordsAcetates, Cell-Free System, Cloning, Molecular, Genes, Fungal, Promoter Regions, Genetic, Regulatory Sequences, Nucleic Acid, RNA Polymerase II, Saccharomyces cerevisiae, Templates, Genetic, Transcription Factors, Transcription, Genetic
Abstract

A yeast nuclear extract supported transcription from the CYC1 and PYK1 promoters. Transcription was initiated in vitro at or near sites used in vivo. Deletion of "TATA" sequences abolished the reaction. alpha-Amanitin (10 micrograms/ml) and chloride (100 mM) were highly inhibitory.

DOI10.1073/pnas.84.24.8839
Alternate JournalProc Natl Acad Sci U S A
PubMed ID3321057
PubMed Central IDPMC299646
Grant ListGM-36659 / GM / NIGMS NIH HHS / United States

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