Submitted by jom4013 on December 3, 2020 - 4:18pm
| Title | Accurate initiation at RNA polymerase II promoters in extracts from Saccharomyces cerevisiae. |
| Publication Type | Journal Article |
| Year of Publication | 1987 |
| Authors | Lue NF, Kornberg RD |
| Journal | Proc Natl Acad Sci U S A |
| Volume | 84 |
| Issue | 24 |
| Pagination | 8839-43 |
| Date Published | 1987 Dec |
| ISSN | 0027-8424 |
| Keywords | Acetates, Cell-Free System, Cloning, Molecular, Genes, Fungal, Promoter Regions, Genetic, Regulatory Sequences, Nucleic Acid, RNA Polymerase II, Saccharomyces cerevisiae, Templates, Genetic, Transcription Factors, Transcription, Genetic |
| Abstract | A yeast nuclear extract supported transcription from the CYC1 and PYK1 promoters. Transcription was initiated in vitro at or near sites used in vivo. Deletion of "TATA" sequences abolished the reaction. alpha-Amanitin (10 micrograms/ml) and chloride (100 mM) were highly inhibitory. |
| DOI | 10.1073/pnas.84.24.8839 |
| Alternate Journal | Proc Natl Acad Sci U S A |
| PubMed ID | 3321057 |
| PubMed Central ID | PMC299646 |
| Grant List | GM-36659 / GM / NIGMS NIH HHS / United States |